Riemenschneider Jenny, Garrison Aura, Geisbert Joan, Jahrling Peter, Hevey Michael, Negley Diane, Schmaljohn Alan, Lee John, Hart Mary Kate, Vanderzanden Lorna, Custer David, Bray Mike, Ruff Albert, Ivins Bruce, Bassett Anthony, Rossi Cynthia, Schmaljohn Connie
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.
Vaccine. 2003 Sep 8;21(25-26):4071-80. doi: 10.1016/s0264-410x(03)00362-1.
Multiagent DNA vaccines for highly pathogenic organisms offer an attractive approach for preventing naturally occurring or deliberately introduced diseases. Few animal studies have compared the feasibility of combining unrelated gene vaccines. Here, we demonstrate that DNA vaccines to four dissimilar pathogens that are known biowarfare agents, Bacillus anthracis, Ebola (EBOV), Marburg (MARV), and Venezuelan equine encephalitis virus (VEEV), can elicit protective immunity in relevant animal models. In addition, a combination of all four vaccines is shown to be equally as effective as the individual vaccines for eliciting immune responses in a single animal species. These results demonstrate for the first time the potential of combined DNA vaccines for these agents and point to a possible method of rapid development of multiagent vaccines for disparate pathogens such as those that might be encountered in a biological attack.
针对高致病性生物的多抗原DNA疫苗为预防自然发生或蓄意引入的疾病提供了一种有吸引力的方法。很少有动物研究比较过联合使用不相关基因疫苗的可行性。在此，我们证明，针对四种已知生物战剂病原体（炭疽芽孢杆菌、埃博拉病毒、马尔堡病毒和委内瑞拉马脑炎病毒）的DNA疫苗能够在相关动物模型中引发保护性免疫。此外，在单一动物物种中，四种疫苗联合使用引发免疫反应的效果与单独使用各疫苗的效果相当。这些结果首次证明了联合DNA疫苗针对这些病原体的潜力，并指出了一种可能的方法，可快速开发针对不同病原体的多抗原疫苗，比如可能在生物攻击中遇到的病原体。
