Zhang Qikun, Dai Xiuru, Wang Huanpeng, Wang Fanhua, Tang Dongxue, Jiang Chunyun, Zhang Xiaoyan, Guo Wenjing, Lei Yuanyuan, Ma Changle, Zhang Hui, Li Pinghua, Zhao Yanxiu, Wang Zenglan
Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, China.
State Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University, Tai'an, China.
Front Plant Sci. 2022 Apr 6;13:866063. doi: 10.3389/fpls.2022.866063. eCollection 2022.
Salt stress is an important environmental factor limiting plant growth and crop production. Plant adaptation to salt stress can be improved by chemical pretreatment. This study aims to identify whether hydrogen peroxide (HO) pretreatment of seedlings affects the stress tolerance of seedlings. The results show that pretreatment with HO at appropriate concentrations enhances the salt tolerance ability of Arabidopsis seedlings, as revealed by lower Na levels, greater K levels, and improved K/Na ratios in leaves. Furthermore, HO pretreatment improves the membrane properties by reducing the relative membrane permeability (RMP) and malonaldehyde (MDA) content in addition to improving the activities of antioxidant enzymes, including superoxide dismutase, and glutathione peroxidase. Our transcription data show that exogenous HO pretreatment leads to the induced expression of cell cycle, redox regulation, and cell wall organization-related genes in Arabidopsis, which may accelerate cell proliferation, enhance tolerance to osmotic stress, maintain the redox balance, and remodel the cell walls of plants in subsequent high-salt environments.
盐胁迫是限制植物生长和作物产量的重要环境因素。通过化学预处理可以提高植物对盐胁迫的适应性。本研究旨在确定过氧化氢（H₂O₂）预处理幼苗是否会影响幼苗的胁迫耐受性。结果表明，用适当浓度的H₂O₂预处理可增强拟南芥幼苗的耐盐能力，这表现为叶片中较低的Na⁺水平、较高的K⁺水平以及改善的K⁺/Na⁺比率。此外，H₂O₂预处理除了提高包括超氧化物歧化酶和谷胱甘肽过氧化物酶在内的抗氧化酶活性外，还通过降低相对膜透性（RMP）和丙二醛（MDA）含量来改善膜特性。我们的转录数据表明，外源H₂O₂预处理导致拟南芥中细胞周期、氧化还原调节和细胞壁组织相关基因的诱导表达，这可能会加速细胞增殖、增强对渗透胁迫的耐受性、维持氧化还原平衡，并在随后的高盐环境中重塑植物细胞壁。
