恶臭假单胞菌的多核苷酸磷酸化酶缺陷型突变体。
Polynucleotide phosphorylase-deficient mutants of Pseudomonas putida.
作者信息
Favaro Rebecca, Dehò Gianni
机构信息
Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy.
出版信息
J Bacteriol. 2003 Sep;185(17):5279-86. doi: 10.1128/JB.185.17.5279-5286.2003.
Abstract
In bacteria, polynucleotide phosphorylase (PNPase) is one of the main exonucleolytic activities involved in RNA turnover and is widely conserved. In spite of this, PNPase does not seem to be essential for growth if the organisms are not subjected to special conditions, such as low temperature. We identified the PNPase-encoding gene (pnp) of Pseudomonas putida and constructed deletion mutants that did not exhibit cold sensitivity. In addition, we found that the transcription pattern of pnp upon cold shock in P. putida was markedly different from that in Escherichia coli. It thus appears that pnp expression control and the physiological roles in the cold may be different in different bacterial species.
摘要
在细菌中，多核苷酸磷酸化酶（PNPase）是参与RNA周转的主要核酸外切酶活性之一，并且广泛存在。尽管如此，如果生物体不处于特殊条件下，如低温，PNPase似乎对生长不是必需的。我们鉴定了恶臭假单胞菌的PNPase编码基因（pnp），并构建了不表现出冷敏感性的缺失突变体。此外，我们发现恶臭假单胞菌在冷休克时pnp的转录模式与大肠杆菌中的明显不同。因此，不同细菌物种中pnp的表达调控及其在低温下的生理作用可能有所不同。
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