Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, EAN, Oeiras, 2780-157, Portugal.
Microb Biotechnol. 2021 Nov;14(6):2316-2333. doi: 10.1111/1751-7915.13890. Epub 2021 Aug 24.
The development of synthetic biology has brought an unprecedented increase in the number molecular tools applicable into a microbial chassis. The exploration of such tools into different bacteria revealed not only the challenges of context dependency of biological functions but also the complexity and diversity of regulatory layers in bacterial cells. Most of the standardized genetic tools and principles/functions have been mostly based on model microorganisms, namely Escherichia coli. In contrast, the non-model pseudomonads lack a deeper understanding of their regulatory layers and have limited molecular tools. They are resistant pathogens and promising alternative bacterial chassis, making them attractive targets for further studies. Ribonucleases (RNases) are key players in the post-transcriptional control of gene expression by degrading or processing the RNA molecules in the cell. These enzymes act according to the cellular requirements and can also be seen as the recyclers of ribonucleotides, allowing a continuous input of these cellular resources. This makes these post-transcriptional regulators perfect candidates to regulate microbial physiology. This review summarizes the current knowledge and unique properties of ribonucleases in the world of pseudomonads, taking into account genomic context analysis, biological function and strategies to use ribonucleases to improve biotechnological processes.
合成生物学的发展带来了微生物底盘中适用的分子工具数量的空前增加。这些工具在不同细菌中的探索不仅揭示了生物功能的上下文依赖性的挑战，还揭示了细菌细胞中调控层的复杂性和多样性。大多数标准化的遗传工具和原理/功能主要基于模式微生物，即大肠杆菌。相比之下，非模式假单胞菌对其调控层的了解有限，并且分子工具也很有限。它们是具有抗性的病原体，也是有前途的替代细菌底盘，这使得它们成为进一步研究的有吸引力的目标。核糖核酸酶（RNases）是通过降解或加工细胞中的 RNA 分子来控制基因表达的转录后调控的关键因子。这些酶根据细胞的需求发挥作用，也可以被视为核糖核苷酸的回收者，允许这些细胞资源的持续输入。这使得这些转录后调控因子成为调节微生物生理学的理想候选物。本综述考虑到基因组背景分析、生物学功能以及利用核糖核酸酶来改进生物技术过程的策略，总结了假单胞菌中核糖核酸酶的现有知识和独特性质。
