转染和细胞内运输过程中DNA复合物的超微结构分析。
Ultrastructural analysis of DNA complexes during transfection and intracellular transport.
作者信息
Cartier Régis, Velinova Maria, Lehman Cathleen, Erdmann Bettina, Reszka Regina
机构信息
Max-Delbrück Center for Molecular Medicine, Berlin, Germany.
出版信息
J Histochem Cytochem. 2003 Sep;51(9):1237-40. doi: 10.1177/002215540305100914.
We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2-1-micro m aggregates composed of 30-50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates.
我们提出了一种基于透射电子显微镜的简单方法，该方法无需使用标记DNA即可研究多聚体介导的转染早期步骤。超微结构分析显示，由30-50纳米亚基组成的0.2-1微米聚集体被内化。此外，还揭示了内化过程的新细节，表明大DNA聚集体存在非特异性细胞进入机制。
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