Sounigo Olivier, Lachenaud Philippe, Bastide Philippe, Cilas Christian, N'Goran Jeanne, Lanaud Claire
CIRAD BP 5035 34032 Montpellier, France.
J Appl Genet. 2003;44(3):339-53.
In order to evaluate twelve doubled haploids (DHs) of Theobroma cacao L. used as parents, a trial was set up in Côte d'Ivoire. Several traits were observed, such as yield, vigour, yield/vigour ratios, resistance to the black pod disease caused by Phytophthora, percentage of flat beans and mean weight of 100 cocoa beans. Out of the three progenies derived from crosses between two DHs, two showed severe drawbacks. A reduction of the heterogeneity within these progenies was occasionally observed for some of the traits, but failed to be consistent. When tested as female parents in combination with diploid testers, some of the DHs showea significantly higher combining value than their parents for traits such as the mean weight of 100 beans and the yield/canopy surface ratio. The results showed the potential of DHs to improve selected parents in only one cycle of selection but more crosses between two DHs need to be tested in order to evaluate potential of the resulting F(1) progenies.
为了评估用作亲本的12个可可树双单倍体（DHs），在科特迪瓦进行了一项试验。观察了几个性状，如产量、活力、产量/活力比、对疫霉引起的黑荚病的抗性、扁平豆的百分比和100颗可可豆的平均重量。在两个DHs杂交产生的三个后代中，有两个表现出严重缺陷。偶尔会观察到这些后代中某些性状的异质性有所降低，但并不一致。当与二倍体测试者作为母本进行测试时，一些DHs在100颗豆子的平均重量和产量/树冠表面积比等性状上表现出比其亲本显著更高的配合力。结果表明，DHs有潜力在仅一个选择周期内改良选定的亲本，但需要测试更多两个DHs之间的杂交组合，以评估所得F(1)后代的潜力。
