Bocianowski Jan, Chełkowski Jerzy, Kuczyńska Anetta, Wiśniewska Halina, Surma Maria, Adamski Tadeusz
Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60479 Poznań, Poland.
J Appl Genet. 2003;44(3):355-60.
Thirty doubled haploid (DH) lines of barley derived from F(1) of a cross between the six-rowed cultivar Pomo and two-rowed cultivar Maresi were examined for susceptibility to Fusarium seedling blight (SB) and head blight (FHB), measured by mycotoxin (nivalenol) content of kernels. RAPD (random amplified polymorphic DNA) polymorphism was analysed by using 53 decamer primers. Amplification products (APs) were 200 bp up to 2000 bp in size on average 5.7 per primer and the total number of APs was 284, 51.06% of which were polymorphic. Only 32 APs differentiated the examined DH lines - 19 APs for nivalenol content of kernels and 13 for seedling resistance. DH lines segregated with continuous distribution of resistance to FHB and SB. At the seedling stage all DH lines exhibited lower susceptibility than parental cultivars, but in the adult stage only two lines (MP 2 and MP 7) appeared to be more resistant to FHB, i.e. accumulated in kernels a lower amount of mycotoxin than cultivars Maresi and Pomo.
对由六行品种波莫（Pomo）和两行品种马雷西（Maresi）杂交所得F(1)衍生的30个大麦双单倍体（DH）系进行了镰刀菌幼苗枯萎病（SB）和穗腐病（FHB）易感性检测，通过籽粒中霉菌毒素（雪腐镰刀菌烯醇）含量来衡量。使用53个十聚体引物分析了随机扩增多态性DNA（RAPD）多态性。扩增产物（APs）大小在200 bp至2000 bp之间，每个引物平均有5.7个，APs总数为284个，其中51.06%具有多态性。仅有32个APs可区分所检测的DH系——19个与籽粒中雪腐镰刀菌烯醇含量有关，13个与幼苗抗性有关。DH系在对FHB和SB的抗性上呈连续分布。在幼苗期，所有DH系的易感性均低于亲本品种，但在成株期，只有两个系（MP 2和MP 7）对FHB表现出更强抗性，即籽粒中积累的霉菌毒素量低于马雷西和波莫品种。
