Warzecha Tomasz, Skrzypek Edyta, Adamski Tadeusz, Surma Maria, Kaczmarek Zygmunt, Sutkowska Agnieszka
University of Agriculture in Kraków, Department of Plant Breeding and Seed Science Łobzowska 24, 31-140 Kraków, Poland.
Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
Plant Pathol J. 2019 Apr;35(2):112-124. doi: 10.5423/PPJ.OA.07.2018.0124. Epub 2019 Apr 1.
Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the species The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to according to direct parameters: disease rating (DR), fresh weight of leaves and roots, kernel weight per spike, kernel number per spike, plump kernels, and indirect parameters - chlorophyll fluorescence (CF). Plate assay, greenhouse and field tests were performed on 30 spring barley doubled haploid (DH) lines and their parents infected with . Direct parameters proved that hulled genotypes show less symptoms. Most studied chlorophyll fluorescence (CF) parameters (apart from DIo/CS - amount of energy dissipated from PSII for laboratory test, TRo/CS - amount of excitation energy trapped in PSII reaction centers, ETo/CS - amount of energy used for electron transport and RC/CS - number of active reaction centres in the state of fully reduced PSII reaction center in field experiment) were significantly affected by infection. In all experiments, hulled genotypes had higher values of CF parameters compared to hull-less ones. Significant correlations were detected between direct and indirect parameters and also between various environments. It was revealed that ABS/CS, TRo/CS, and RC/CS have significant positive correlation in greenhouse test and field experiment. Significant correlations suggest the possibility of applying the CF parameters in selection of barley DH lines resistant to infection.
全球大麦受到由该物种引起的镰刀菌幼苗枯萎病（FSB）和镰刀菌穗腐病（FHB）的严重影响。本研究的目的是根据直接参数：病情评级（DR）、叶和根的鲜重、每穗粒重、每穗粒数、饱满籽粒，以及间接参数——叶绿素荧光（CF），来促进有壳和无壳大麦在不同生长阶段对[病原菌名称未给出]的抗性。对30个春大麦双单倍体（DH）品系及其感染[病原菌名称未给出]的亲本进行了平板测定、温室和田间试验。直接参数证明有壳基因型表现出较少的症状。大多数研究的叶绿素荧光（CF）参数（除了实验室试验中从PSII消散的能量DIo/CS、PSII反应中心捕获的激发能TRo/CS、用于电子传递的能量ETo/CS以及田间试验中完全还原的PSII反应中心状态下的活性反应中心数量RC/CS）受到[病原菌名称未给出]感染的显著影响。在所有试验中，有壳基因型的CF参数值高于无壳基因型。在直接和间接参数之间以及不同环境之间检测到显著相关性。结果表明，在温室试验和田间试验中，ABS/CS、TRo/CS和RC/CS具有显著正相关。显著相关性表明在选择抗[病原菌名称未给出]感染的大麦DH品系时应用CF参数的可能性。
