Gentzel Marc, Köcher Thomas, Ponnusamy Saravanan, Wilm Matthias
European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Proteomics. 2003 Aug;3(8):1597-610. doi: 10.1002/pmic.200300486.
Liquid chromatography tandem mass spectrometry is a major tool for identifying proteins. The fragment spectra of peptides can be interpreted automatically in conjunction with a sequence database search. With the development of powerful automatic search engines, research now focuses on optimizing the result returned from database searches. We present a series of preprocessing steps for fragment spectra to increase the accuracy and specificity of automatic database searches. After processing, the correct amino acid sequences from the database can be related better to the fragment spectra. This increases the sensitivity and reliability of protein identifications, especially with very large genomic databanks, and can be important for the systematic characterization of post-translational modifications.
液相色谱串联质谱法是鉴定蛋白质的主要工具。肽段的碎片谱可结合序列数据库搜索进行自动解读。随着强大的自动搜索引擎的发展，目前的研究重点是优化数据库搜索返回的结果。我们提出了一系列针对碎片谱的预处理步骤，以提高自动数据库搜索的准确性和特异性。处理后，数据库中正确的氨基酸序列能更好地与碎片谱相关联。这提高了蛋白质鉴定的灵敏度和可靠性，特别是对于非常大的基因组数据库而言，并且对于翻译后修饰的系统表征可能很重要。
