Sun Yuan-Dong, Liu Shao-Jun, Zhang Chun, Li Jian-Zhong, Huang Wei-Ren, Zhang Jian, Luo Kai-Kun, Zhou Gong-Jian, Liu Yun
Key Lab of Protein Chemistry and Developmental Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.
Yi Chuan Xue Bao. 2003 May;30(5):414-8.
In this paper, the chromosome number of F9-F11 hybridis of Red crucian (Carassius auratus) x common carp(Cryprinus carpio) was examined by means of kidney cell-PHA culture. The results showed that F9-F11 allotetraploids possessed 200 chromosomes, with the karyotype formula 44 m + 68 sm + 44 st + 44 t, which were the same as the data indicated in F3-F8 allotetraploids. Both female and male of F9-F11 allotetraploids had the normal ovaries and testes that were able to produce the normal dipoid ova and sperm. In nature conditions, without the injection of the extraneous hormone, the females and males of F9-F11 allotetraploids mated each other to produce new generation of tetraploids. With the stable genetic tetraploidy and the fertility in the nature environments, this allotetraploid population presented the key factors to form a new species with 200 chromosomes.
本文采用肾细胞 - PHA培养法对红鲫（Carassius auratus）与鲤鱼（Cryprinus carpio）杂交F9 - F11代的染色体数目进行了检测。结果显示，F9 - F11代异源四倍体拥有200条染色体，核型公式为44m + 68sm + 44st + 44t，这与F3 - F8代异源四倍体的数据一致。F9 - F11代异源四倍体的雌性和雄性均具有正常的卵巢和精巢，能够产生正常的二倍体卵子和精子。在自然条件下，无需注射外源激素，F9 - F11代异源四倍体的雌雄个体相互交配就能产生新一代的四倍体。由于具有稳定的遗传四倍性且在自然环境中可育，这个异源四倍体群体具备了形成一个拥有200条染色体新物种的关键因素。
