四倍体棉花品种产量和纤维品质性状的数量性状基因座定位

Localization of QTLs for yield and fiber quality traits of tetraploid cotton cultivar.

作者信息

Wu Mao-Qing, Zhang Xian-Long, Nie Yi-Chun, He Dao-Hua

机构信息

National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China.

出版信息

Yi Chuan Xue Bao. 2003 May;30(5):443-52.

PMID:12924159

Abstract

Using SSR and RAPD as molecular markers, and the 69 F2 families from a cross between Handan208 (Gossypium hirsutum) and Pima90 (Gossypium barbadense) as a mapping population, a linkage map comprising 126 markers was constructed. With an average spacing 13.7 cM between markers, the linkage map spanned 1,717.0 cM, which covers approximately 34.34% of the total recombinational length of cotton genome. Based on the linkage map and the F2:3 phenotypic data, overall genome QTL screening was conducted by composite interval mapping method, then 29 QTLs related to cotton yield traits and fiber quality traits were found. There are 16 loci related to yield traits and 13 loci related to fiber quality. Particularly, two QTLs relevant to fiber strength were detected, which could explain phenotypic variance of 34.15% and 13.86%, respectively.

摘要

以SSR和RAPD作为分子标记，以邯郸208（陆地棉）与Pima90（海岛棉）杂交产生的69个F2家系作为作图群体，构建了一张包含126个标记的连锁图谱。标记间平均间距为13.7 cM，连锁图谱跨度为1717.0 cM，约覆盖棉花基因组总重组长度的34.34%。基于该连锁图谱和F2:3表型数据，采用复合区间作图法进行全基因组QTL筛选，共检测到29个与棉花产量性状和纤维品质性状相关的QTL。其中，与产量性状相关的位点有16个，与纤维品质相关的位点有13个。特别地，检测到两个与纤维强度相关的QTL，分别可解释34.15%和13.86%的表型变异。