Wang Min, Li Chengqi, Wang Qinglian
Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100048, People's Republic of China.
J Genet. 2014 Aug;93(2):371-8. doi: 10.1007/s12041-014-0385-9.
Lint percentage is an important character of cotton yield components and it is also correlated with cotton fibre development. In this study, we used a high lint percentage variety, Baimian1, and a low lint percentage, TM-1 genetic standard for Gossypium hirsutum, as parents to construct a mapping populations in upland cotton (G. hirsutum). A quantitative trait locus/loci (QTL) analysis of lint percentage was performed by using two mapping procedures; composite interval mapping (CIM), inclusive composite interval mapping (ICIM) and the F2:3 populations in 2 years. Six main-effect QTL (M-QTL) for lint percentage (four significant and two suggestive) were detected in both years by CIM, and were located on chr. 3, chr. 19, chr. 26 and chr. 5/chr. 19. Of the six QTL, marker intervals and favourable gene sources of the significant M-QTL, qLP-3(2010) and qLP-3(2011) were consistent. These QTL were also detected by ICIM, and therefore, should preferentially be used for markerassisted selection (MAS) of lint percentage. Another M-QTL, qLP-19(2010), was detected by two mapping procedures, and it could also be a candidate for MAS. We detected the interaction between two M-QTL and environment, and 11 epistatic QTL (E-QTL) and their interaction with environment by using ICIM. The study also found two EST-SSRs, NAU1187 and NAU1255, linked to M-QTL for lint percentage that could be candidate markers affecting cotton fibre development.
衣分率是棉花产量构成的一个重要性状，且与棉花纤维发育相关。在本研究中，我们使用衣分率高的品种百棉1号和陆地棉遗传标准系、衣分率低的TM-1作为亲本，构建了陆地棉（陆地棉）作图群体。利用复合区间作图（CIM）、完备复合区间作图（ICIM）两种作图方法以及两年的F2:3群体，对衣分率进行了数量性状基因座（QTL）分析。通过CIM在两年中均检测到6个衣分率主效QTL（M-QTL）（4个显著QTL和2个推测QTL），它们位于第3染色体、第19染色体、第26染色体以及第5染色体/第19染色体上。在这6个QTL中，显著M-QTL qLP-3(2010)和qLP-3(2011)的标记区间和有利基因源是一致的。这些QTL也通过ICIM检测到，因此应优先用于衣分率的分子标记辅助选择（MAS）。另一个M-QTL qLP-19(2010)通过两种作图方法均被检测到，它也可能是MAS的候选基因。我们检测到两个M-QTL与环境之间的互作，以及通过ICIM检测到11个上位性QTL（E-QTL）及其与环境的互作。该研究还发现两个EST-SSR，NAU1187和NAU1255，与衣分率的M-QTL连锁，可能是影响棉花纤维发育的候选标记。
