Yin Jian-Mei, Wu Yao-Ting, Zhang Jun, Zhang Tian-Zhen, Guo Wang-Zhen, Zhu Xie-Fei
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Sheng Wu Gong Cheng Xue Bao. 2002 Jan;18(2):162-6.
Using interval mapping and marker simple regression methods, the QTLs of yield and its components in (Simian 3 x TM-1) F2 and F2:3, were tagged and Mapped with 39 SSR and 10 RAPD markers having polymorphism between parents screened from 301 pair SSR primers and 1040 RAPD primers. Simian 3 is being grown extensively in Yangtze River cotton-growing valley characterized as high productivity with more bolls and higher lint percent, whereas TM-1, Genetic standard in Upland cotton with more heavy boll weight. In the present report, two QTLs controlling boll size with 18.2% and 21.0% phenotype variance explained in F2:3 generation, one QTL controlling lint percent with 24.9% phenotype variance explained in F2 generation and 5.9% in F2:3 generation and one QTL controlling 100-seed weight with 15.6% phenotype variance explained in F2:3 generation were mapped in Chromosome 9. Additionally, another QTL responsible for 100-seed weight was identified and mapped at the same position in Chromosome 9 in F2:3 generation. It is worth for further to be studied whether it is one QTL for pleiotrophism or two closely linked QTLs. The molecular markers mapped and tagged closely with main QTLs of yield traits in this paper can be used for MAS in cotton high-yield breeding program.
利用区间作图和标记简单回归方法，对（泗棉3号×TM-1）F2和F2:3群体产量及其构成因素的QTL进行了定位。从301对SSR引物和1040条RAPD引物中筛选出在亲本间具有多态性的39个SSR标记和10个RAPD标记进行分析。泗棉3号在长江流域棉区广泛种植，具有高产、结铃多、衣分高的特点；而TM-1是陆地棉的遗传标准系，铃重较大。本研究中，在F2:3代定位到2个控制铃大小的QTL，表型变异解释率分别为18.2%和21.0%；在F2代定位到1个控制衣分的QTL，表型变异解释率为24.9%，在F2:3代表型变异解释率为5.9%；在F2:3代定位到1个控制百粒重的QTL，表型变异解释率为15.6%，这些QTL均位于第9染色体上。此外，在F2:3代还在第9染色体的相同位置鉴定并定位到另一个控制百粒重的QTL。该QTL是一因多效还是两个紧密连锁的QTL，值得进一步研究。本文定位并紧密连锁于产量性状主QTL的分子标记可用于棉花高产育种的分子标记辅助选择。
