Césarini J P, Michel L, Maurette J M, Adhoute H, Béjot M
Fondation Ophtalmologique A de Rothschild, Paris, France.
Photodermatol Photoimmunol Photomed. 2003 Aug;19(4):182-9. doi: 10.1034/j.1600-0781.2003.00044.x.
BACKGROUND/AIMS: The ultraviolet (UV) portion of sunlight is involved in the induction and development of skin cancers against which a limited photoprotection may be provided by reduced time of exposure, clothing, and sunscreen applications. The concept of an effective, safe, systemic photoprotection will circumvent many of the shortcomings. The UV-induced oxidative stress is a cause of DNA damage and a few publications have shown, in humans, minimal benefits, if any, of the oral intake of antioxidant complex, contrasting with the large literature showing beneficial effects in vitro or in animal models.
We investigated, in 25 healthy individuals, the capacity of an antioxidant complex (AOC) - vitamins (lycopene, beta-carotene, alpha-tocopherol), selenium - to reduce UV-induced damages. The AOC was administered orally, daily during 7 weeks. Before and after irradiations, before and after the intake of the product, six parameters were studied: skin color by chromametry, minimal erythemal dose and, on skin biopsies, sunburn cells (SBCs), p53 detected by immunohistochemistry, pigmentation index, and levels of lipoperoxides (thiobarbituric acid reaction).
After the oral intake of AOC, we observed an elevation of the actinic erythema threshold (+20%, P=0.01) and a general reduction of the UV-induced erythemas, a reduction of the UV-induced p53 expression (P<0.05) and of SBCs (P<0.01), and a parallel reduction of the lipoperoxide levels (P<0.01). The pigmentation was increased (P<0.01).
After the oral intake of an antioxidant complex, many parameters of the epidermal defense against UV-induced damages are significantly improved. The oral intake of AOC could provide a safe, daylong and efficient complement to photo-protective measures provided by topical and physical agents and may contribute to reduce the DNA damages leading to skin aging and skin cancers.
背景/目的：阳光中的紫外线（UV）参与皮肤癌的诱发和发展，减少暴露时间、穿着衣物和涂抹防晒霜可能提供有限的光保护作用。有效、安全的系统性光保护概念将规避许多缺点。紫外线诱导的氧化应激是DNA损伤的一个原因，一些研究表明，在人类中，口服抗氧化复合物的益处微乎其微，这与大量在体外或动物模型中显示有益效果的文献形成对比。
我们在25名健康个体中研究了一种抗氧化复合物（AOC）——维生素（番茄红素、β-胡萝卜素、α-生育酚）、硒——减少紫外线诱导损伤的能力。AOC每天口服，持续7周。在照射前后、服用产品前后，研究了六个参数：通过色度法测定的皮肤颜色、最小红斑剂量，以及在皮肤活检中，晒伤细胞（SBCs）、通过免疫组织化学检测的p53、色素沉着指数和脂过氧化物水平（硫代巴比妥酸反应）。
口服AOC后，我们观察到光化性红斑阈值升高（+20%，P = 0.01），紫外线诱导的红斑普遍减少，紫外线诱导的p53表达降低（P < 0.05）和SBCs减少（P < 0.01），同时脂过氧化物水平平行降低（P < 0.01）。色素沉着增加（P < 0.01）。
口服抗氧化复合物后，表皮抵御紫外线诱导损伤的许多参数得到显著改善。口服AOC可为局部和物理防晒措施提供安全、全天且有效的补充，并可能有助于减少导致皮肤衰老和皮肤癌的DNA损伤
