Kallmes D F, Williams A D, Cloft H J, Lopes M B, Hankins G R, Helm G A
Department of Radiology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Radiology. 1998 May;207(2):519-23. doi: 10.1148/radiology.207.2.9577504.
To demonstrate in vivo that platinum embolic coils can be used to deliver genetically modified, growth factor-secreting fibroblast grafts into the endovascular space with the long-term goal of improving fibrosis within coil-embolized cerebral aneurysms.
Murine fibroblasts that contained multiple inserts of the DNA for human basic fibroblast growth factor were grown in culture onto 10-mm-long segments of Guglielmi detachable coils. Control (n = 4) and fibroblast-bearing (n = 4) coils were implanted into the common carotid artery in nude rats. The arterial segments that contained the coil were harvested after 14 or 35 days. Cellular content and collagen formation in the treated vessels were assessed histologically.
At both 14 and 35 days, samples with control coils showed primarily involuting blood elements with minimal fibroblast proliferation or collagen formation. At 14 days, samples with fibroblast-bearing coils showed extensive fibroblast proliferation. At 35 days, samples with fibroblast-bearing coils showed marked interval fibroblast proliferation and collagen formation.
Platinum coils can be used as a cell delivery device. Direct intravascular implantation of growth factor-secreting fibroblast grafts leads to improved intravascular scar formation, therefore theoretically reducing the potential for aneurysm regrowth or coil compaction.
在体内证明铂栓塞线圈可用于将经基因改造的、分泌生长因子的成纤维细胞移植物输送到血管内空间，其长期目标是改善线圈栓塞的脑动脉瘤内的纤维化。
将含有多个人类碱性成纤维细胞生长因子DNA插入片段的小鼠成纤维细胞在培养物中生长到10毫米长的Guglielmi可脱卸线圈片段上。将对照线圈（n = 4）和携带成纤维细胞的线圈（n = 4）植入裸鼠的颈总动脉。在14天或35天后收获含有线圈的动脉节段。对治疗血管中的细胞含量和胶原形成进行组织学评估。
在14天和35天时，含对照线圈的样本主要显示血液成分退化，成纤维细胞增殖或胶原形成极少。在14天时，含成纤维细胞线圈的样本显示广泛的成纤维细胞增殖。在35天时，含成纤维细胞线圈的样本显示明显的间隔成纤维细胞增殖和胶原形成。
铂线圈可作为细胞递送装置。直接血管内植入分泌生长因子的成纤维细胞移植物可改善血管内瘢痕形成，因此理论上可降低动脉瘤再生长或线圈压缩的可能性。
