Wu Hongkun, Fan Mingwen, Zhou Xuedong, Mo Anchun, Bian Zhuan, Zhang Qi, Chen Zhi
Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei, PR China.
Caries Res. 2003 Sep-Oct;37(5):374-80. doi: 10.1159/000072171.
Streptococcus mutans and Streptococcus sobrinus are associated with the development of dental caries. The purpose of this study was to differentiate these bacteria by morphology, biochemical characteristics and PCR, and to compare their occurrence with the prevalence of dental caries in the Mosuo people. Plaque samples were collected from the permanent first molar in 126 Mosuo people (83 females, 43 males, aged 25-55 years, average age 36.1 +/- 7.73). Dental status was recorded as DMFT by WHO caries diagnostic criteria. Males had a significantly lower prevalence of caries and DMFT than females: 11.4 vs. 86.9% and 1.65 vs. 6.95, respectively (p<0.001). Morphological and biochemical tests gave unreliable results. The prevalence of S. mutans and S. sobrinus was 75.4 and 57.1%, respectively. 26.5% of females and 53.5% of males were positive for S. mutans alone, 18.1% of females and 16.3% of males were positive for S. sobrinus alone, while 50.6% of females and 18.6% of males were positive for both S. mutans and S. sobrinus and 4.8% of females and 11.6% of males were negative for both species. The DMFT scores of subjects positive for both S. mutans and S. sobrinus were significantly higher than of those positive for either S. mutans or S. sobrinus alone. These results indicate that subjects harboring both S. mutans and S. sobrinus have a significantly higher prevalence of dental caries than those with S. mutans or S. sobrinus alone.
变形链球菌和远缘链球菌与龋齿的发生有关。本研究的目的是通过形态学、生化特征和聚合酶链反应（PCR）来区分这些细菌，并比较它们的检出情况与摩梭人龋齿患病率的关系。从126名摩梭人（83名女性，43名男性，年龄在25 - 55岁之间，平均年龄36.1±7.73岁）的恒牙第一磨牙采集菌斑样本。根据世界卫生组织的龋齿诊断标准，将牙齿状况记录为龋失补牙数（DMFT）。男性的龋齿患病率和DMFT显著低于女性：分别为11.4%对86.9%以及1.65对6.95（p<0.001）。形态学和生化测试结果不可靠。变形链球菌和远缘链球菌的患病率分别为75.4%和57.1%。仅变形链球菌阳性的女性占26.5%，男性占53.5%；仅远缘链球菌阳性的女性占18.1%，男性占16.3%；而变形链球菌和远缘链球菌均阳性的女性占50.6%，男性占18.6%；两种菌均阴性的女性占4.8%，男性占11.6%。变形链球菌和远缘链球菌均阳性的受试者的DMFT评分显著高于仅变形链球菌或仅远缘链球菌阳性的受试者。这些结果表明，同时携带变形链球菌和远缘链球菌的受试者的龋齿患病率显著高于仅携带变形链球菌或仅携带远缘链球菌的受试者。
