Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil.
J Appl Oral Sci. 2010 Jul-Aug;18(4):426-31. doi: 10.1590/s1678-77572010000400018.
The aim of this study was to detect the prevalence of selected bacterial species in intraoral sites of patients with chronic periodontitis (CP) using multiplex polymerase chain reaction (PCR).
Samples were collected from the tongue dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients with untreated CP. Multiplex PCR was used to determine prevalence rates, which were then compared using a chi-square test. Significance level was set at p<0.05. Mean and standard deviation values were used to evaluate variations in prevalence according to site.
The prevalence of S. mutans was 70% in saliva; 60% in samples collected from the tongue dorsum; 50% in samples collected from the buccal mucosa; 56.5% in the supragingival plaque; and 53.5% in the subgingival plaque. The prevalence of E. faecalis ranged from 3.5% to 13.5% in all intraoral microenvironment. The highest prevalence of P. gingivalis was found in subgingival plaque (53.5%), and of P. intermedia in supragingival plaque (33.5%), subgingival plaque (30%) and tongue dorsum (33.5%). The prevalence of bacteria did not vary significantly among the intraoral sites.
All studied bacteria were identified in intraoral sites. S. mutans, P. gingivalis and P. intermedia had high prevalence rates, but the prevalence of E. faecalis was low. Multiplex PCR proved to be an adequate method for epidemiological studies.
本研究旨在使用多重聚合酶链反应（PCR）检测慢性牙周炎（CP）患者口腔内选定细菌种属的流行情况。
采集 30 例未经治疗的 CP 患者的舌背、颊黏膜、龈上和龈下菌斑和唾液样本。使用多重 PCR 确定流行率，然后使用卡方检验进行比较。设定显著性水平为 p<0.05。使用均值和标准差值根据部位评估流行率的变化。
变形链球菌在唾液中的流行率为 70%；舌背采集样本中为 60%；颊黏膜采集样本中为 50%；龈上菌斑中为 56.5%；龈下菌斑中为 53.5%。粪肠球菌在所有口腔微环境中的流行率为 3.5%至 13.5%。牙龈卟啉单胞菌在龈下菌斑中的流行率最高（53.5%），中间普氏菌在龈上菌斑（33.5%）、龈下菌斑（30%）和舌背（33.5%）中的流行率最高。细菌在口腔内各部位的流行率无显著差异。
所有研究的细菌均在口腔内各部位检出。变形链球菌、牙龈卟啉单胞菌和中间普氏菌的流行率较高，但粪肠球菌的流行率较低。多重 PCR 被证明是一种用于流行病学研究的适当方法。
