Schultz I J, Kiemeney L A, Witjes J A, Schalken J A, Willems J L, Swinkels D W, de Kok J B
Department of Clinical Chemistry/564, University Medical Center Nijmegen, PO Box 9101, 6500 HB, Nijmegen, The Netherlands.
Anticancer Res. 2003 Jul-Aug;23(4):3327-31.
Expression of the inhibitor of apoptosis protein survivin is up-regulated in many tumors of epithelial origin and frequently shows a relationship with disease prognosis.
We investigated survivin mRNA expression in 32 urothelial cell carcinomas by use of real-time quantitative PCR. Expression values were normalized to transcript levels of the housekeeping gene cyclophilin.
All bladder tumor tissues expressed survivin mRNA. The median normalized survivin mRNA expression values were 0.26 for superficial tumors (n = 17) and 0.78 for invasive tumors (n = 15). A significant relationship with increasing pathological stage (p < 0.001) and grade (p < 0.001) was observed. Although survivin mRNA expression did not relate to disease progression or the patient survival period, patients with superficial bladder tumors and normalized survivin values over 0.26 had an increased risk of recurrence (log-rank test: p = 0.018).
Our results suggest that quantitative measurement of survivin mRNA 1) can identify invasive and high-grade urothelial cell carcinomas and 2) may be used as an indicator for early recurrence of superficial tumors.
凋亡抑制蛋白survivin在许多上皮源性肿瘤中表达上调，且常与疾病预后相关。
我们运用实时定量PCR技术研究了32例尿路上皮细胞癌中survivin mRNA的表达情况。将表达值标准化为管家基因亲环素的转录水平。
所有膀胱肿瘤组织均表达survivin mRNA。浅表性肿瘤（n = 17）的标准化survivin mRNA表达值中位数为0.26，浸润性肿瘤（n = 15）为0.78。观察到其与病理分期增加（p < 0.001）和分级增加（p < 0.001）存在显著相关性。虽然survivin mRNA表达与疾病进展或患者生存期无关，但浅表性膀胱肿瘤且标准化survivin值超过0.26的患者复发风险增加（对数秩检验：p = 0.018）。
我们的结果表明，survivin mRNA的定量检测1）可识别浸润性和高级别尿路上皮细胞癌，2）可作为浅表性肿瘤早期复发的指标。
