存活素信使核糖核酸表达在恶性尿路上皮细胞癌中升高，并可预测复发时间。

Survivin mRNA expression is elevated in malignant urothelial cell carcinomas and predicts time to recurrence.

作者信息

Schultz I J, Kiemeney L A, Witjes J A, Schalken J A, Willems J L, Swinkels D W, de Kok J B

机构信息

Department of Clinical Chemistry/564, University Medical Center Nijmegen, PO Box 9101, 6500 HB, Nijmegen, The Netherlands.

出版信息

Anticancer Res. 2003 Jul-Aug;23(4):3327-31.

PMID:12926071

Abstract

BACKGROUND

Expression of the inhibitor of apoptosis protein survivin is up-regulated in many tumors of epithelial origin and frequently shows a relationship with disease prognosis.

MATERIALS AND METHODS

We investigated survivin mRNA expression in 32 urothelial cell carcinomas by use of real-time quantitative PCR. Expression values were normalized to transcript levels of the housekeeping gene cyclophilin.

RESULTS

All bladder tumor tissues expressed survivin mRNA. The median normalized survivin mRNA expression values were 0.26 for superficial tumors (n = 17) and 0.78 for invasive tumors (n = 15). A significant relationship with increasing pathological stage (p < 0.001) and grade (p < 0.001) was observed. Although survivin mRNA expression did not relate to disease progression or the patient survival period, patients with superficial bladder tumors and normalized survivin values over 0.26 had an increased risk of recurrence (log-rank test: p = 0.018).

CONCLUSION

Our results suggest that quantitative measurement of survivin mRNA 1) can identify invasive and high-grade urothelial cell carcinomas and 2) may be used as an indicator for early recurrence of superficial tumors.

摘要

背景

凋亡抑制蛋白survivin在许多上皮源性肿瘤中表达上调，且常与疾病预后相关。

材料与方法

我们运用实时定量PCR技术研究了32例尿路上皮细胞癌中survivin mRNA的表达情况。将表达值标准化为管家基因亲环素的转录水平。

结果

所有膀胱肿瘤组织均表达survivin mRNA。浅表性肿瘤（n = 17）的标准化survivin mRNA表达值中位数为0.26，浸润性肿瘤（n = 15）为0.78。观察到其与病理分期增加（p < 0.001）和分级增加（p < 0.001）存在显著相关性。虽然survivin mRNA表达与疾病进展或患者生存期无关，但浅表性膀胱肿瘤且标准化survivin值超过0.26的患者复发风险增加（对数秩检验：p = 0.018）。