Liccardi G, Dente B, Triggiani M, Russo M, Diamare F, Massari A, Pinzarrone R, D'Isanto R, Letizia M, D'Amato M, D'Amato G
Division of Pneumology and Allergology, A. Cardarelli Hospital, Naples, Italy.
J Investig Allergol Clin Immunol. 2002;12(4):235-41.
The evaluation of specific IgE by using appropriate immunoassays represents a useful alternative diagnostic procedure where skin prick tests (SPTs) are not conclusive in clarifying the etiological role of suspected allergens. This study compares the results of the evaluation of specific IgE by using the CARLA system vs. other commercially available immunoassays (CAP system, Ala-STAT Medical system, ALLERgen IFCI Clone System) carried out on the same blood samples obtained from allergic/SPTs negative patients and vs. SPTs. We evaluated serum specific IgE produced against five selected allergens (Dermatophagoides pteronyssinus, Olea europaea, Parietaria judaica, Lolium perenne and Phleum pratense) by using these immunoassays and the correlations between the results of SPTs and IgE evaluations. We demonstrated a good correlation between these last parameters including a high degree of sensitivity and specificity. The reproducibility of the CARLA system was very high by comparing the results obtained by two different laboratories. The results of the CARLA system were well correlated to those of other well-known immunoassays such as CAP system and Ala STAT system. In conclusion, the CARLA system represents an efficient and reliable immunoassay for the evaluation of serum specific IgE.
当皮肤点刺试验（SPT）在明确可疑变应原的病因学作用方面不能得出结论时，采用适当的免疫测定法评估特异性IgE是一种有用的替代诊断方法。本研究比较了使用CARLA系统与其他市售免疫测定法（CAP系统、Ala-STAT医学系统、ALLERgen IFCI克隆系统）对从过敏/SPT阴性患者采集的相同血液样本进行特异性IgE评估的结果，并与SPT结果进行了比较。我们使用这些免疫测定法评估了针对五种选定变应原（屋尘螨、油橄榄、墙草、多年生黑麦草和梯牧草）产生的血清特异性IgE，以及SPT结果与IgE评估结果之间的相关性。我们证明了这些最后参数之间具有良好的相关性，包括高度的敏感性和特异性。通过比较两个不同实验室获得的结果，CARLA系统的可重复性非常高。CARLA系统的结果与其他知名免疫测定法（如CAP系统和Ala STAT系统）的结果具有良好的相关性。总之，CARLA系统是一种用于评估血清特异性IgE的高效且可靠的免疫测定法。
