Banfi G, Ferrara F, Bonini P, Colombo G, Plebani M, Borghesan F, Faggian D, Caputo M, Andri L, Senna G E
Dipartimento di Medicina di Laboratorio, Istituto Scientifico, H San Raffaele, Milano, Italy.
Eur J Clin Chem Clin Biochem. 1995 Oct;33(10):755-9.
A multicentre trial of Capture Assay Radim Liquid Allergen was performed to define the sensitivity, specificity and clinical reliability of the system in diagnostic allergology. The results of the evaluation were compared with clinical data and in vivo testing. Good agreement was obtained for Dermatophagoides pteronyssinus (D1), Cat's epithelium (E1), Betula verrucosa (T3) and Olea europea (T9), Artemisia vulgaris (W6) and Parietaria officinalis (W19). Some spreading of data was observed for Artemisia absinthium (W5), Cynodon dactylon (G2), and Lolium perenne (G5). We found a high number of negative cases for Alternaria alternata (M6). The advantages offered by the system are the automation, the small quantity of serum requested, the supply of quantitative results in international units of specific IgE, the user-friendly software. The data are sufficiently reliable for the diagnostic system to be introduced into the clinical laboratory allergological routine.
开展了一项关于Capture Assay Radim液体过敏原的多中心试验，以确定该系统在诊断变态反应学中的敏感性、特异性和临床可靠性。将评估结果与临床数据及体内试验进行了比较。对于屋尘螨（D1）、猫上皮（E1）、疣皮桦（T3）、油橄榄（T9）、艾蒿（W6）和墙草（W19），获得了良好的一致性。对于苦艾（W5）、狗牙根（G2）和多年生黑麦草（G5），观察到数据有一定分散。我们发现链格孢（M6）有大量阴性病例。该系统的优点包括自动化、所需血清量少、以特定IgE的国际单位提供定量结果、软件用户友好。这些数据足够可靠，可将该诊断系统引入临床实验室变态反应学常规检测中。
