Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements.
作者信息
Beach Lori, Schweitzer Claude, Scaiano J C
机构信息
Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario, Canada K1N 6N5.
出版信息
Org Biomol Chem. 2003 Feb 7;1(3):450-1. doi: 10.1039/b209284k.
DOI:10.1039/b209284k
PMID:12926241
Abstract
We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss : ds DNA ratio.
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