Kosik-Bogacka Danuta I, Banach Bolesław, Tyrakowski Tomasz, Kozłowska Alina, Kozłowska Sylwia
Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstańców Wielkopolskich 72, PL 70-111 Szczecin, Poland.
Pol J Pharmacol. 2003 Mar-Apr;55(2):213-9.
Effect of selective blockers of sodium and chloride ion transport (amiloride and bumetanide) on transepithelial electrical potential (PD), transepithelial electrical potential difference (dPD), and electrical resistance of the tissue (R) of isolated fragment of the rabbit's caecum were determined using electrophysiological methods designed for measuring ionic currents occurring in epithelial tissues. A modified Ussing apparatus enabling application of mechanical and chemical stimuli on the isolated tissue was used in the experiment. It was demonstrated that amiloride used for incubation of the caecum fragments lowered by some 24% the value of PD and by 50% the value of dPD. Incubation of the caecum fragments with bumetanide resulted in a decrease in the PD value by 73% and in the value of dPD by some 83%. The results obtained with the tissue incubated in Ringer solution with addition of both compounds were comparable with those observed for the tissue incubated with bumetanide. As can be concluded from the above-mentioned experiments, both ion transport pathways contribute jointly to the induction of PD in the epithelium of the rabbits caecum.
采用专门用于测量上皮组织中离子电流的电生理方法，测定了钠和氯离子转运选择性阻滞剂（氨氯吡咪和布美他尼）对兔盲肠离体片段的跨上皮电位（PD）、跨上皮电位差（dPD）和组织电阻（R）的影响。实验中使用了一种改良的Ussing装置，该装置能够对离体组织施加机械和化学刺激。结果表明，用氨氯吡咪孵育盲肠片段可使PD值降低约24%，dPD值降低50%。用布美他尼孵育盲肠片段导致PD值降低73%，dPD值降低约83%。在添加了两种化合物的林格氏液中孵育组织所获得的结果与用布美他尼孵育组织所观察到的结果相当。从上述实验可以得出结论，两种离子转运途径共同促成了兔盲肠上皮中PD的产生。
