Kosik-Bogacka Danuta, Banach Bolesław, Tyrakowski Tomasz
Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstańców Wielkopolskich 72, PL 70-111 Szczecin, Poland.
Pol J Pharmacol. 2003 Mar-Apr;55(2):221-6.
The aim of this study was to determine the effect of starvation on the transport of sodium and chloride ions in the epithelium of rabbit caecum. The experiment consisted in measuring transepithelial electrical potential (PD in mV) and the transepithelial electrical potential difference (dPD in mV) of an isolated fragment of rabbit caecum, before and after 4-day-long starvation. The studied tissue was incubated in Ringer solution and subsequently ion transport was modified through incubation in the Ringer solution supplemented with amiloride or/and bumetanide. It was demonstrated that the values of electrophysiological parameters of the tissue fragments of caecum from starved rabbits were substantially lower than the values for the fragments of control caecum. A similar relationship was observed also in the reaction of this tissue to mechanical stimuli. After the incubation of the caecum tissue fragments in the presence of amiloride or/and bumetanide, the value of transepithelial electrical potential and the sensitivity to mechanical stimuli decreased in both groups studied. Experimental data presented in this paper indicate that the starvation process has effect on lowering sodium and chloride ion transport and decreasing sensitivity of the epithelium of the caecum to mechanical stimuli.
本研究的目的是确定饥饿对兔盲肠上皮中钠和氯离子转运的影响。实验包括测量兔盲肠分离片段在4天饥饿前后的跨上皮电势（以毫伏为单位的PD）和跨上皮电势差（以毫伏为单位的dPD）。将研究的组织在林格氏溶液中孵育，随后通过在添加了氨氯吡咪或/和布美他尼的林格氏溶液中孵育来改变离子转运。结果表明，饥饿兔盲肠组织片段的电生理参数值显著低于对照盲肠片段的值。在该组织对机械刺激的反应中也观察到了类似的关系。在氨氯吡咪或/和布美他尼存在的情况下孵育盲肠组织片段后，所研究的两组中跨上皮电势值和对机械刺激的敏感性均降低。本文给出的实验数据表明，饥饿过程会降低钠和氯离子的转运，并降低盲肠上皮对机械刺激的敏感性。
