Kosik-Bogacka Danuta, Młodzik-Danielewicz Natalia, Banach Bolesław, Tyrakowski Tomasz
Department of Biology and Medical Parasitology, Pomeranian Medical University, Szczecin, Poland.
Postepy Hig Med Dosw (Online). 2005 Jun 3;59:229-35.
The aim of the study was to compare the effects of amiloride and bumetanide on the baseline transepithelial electrical potential difference (PD) and changes in PD during mechanical stimulation (dPD) in isolated cecal and colonic wall of rabbits.
MATERIAL/METHODS: The experiments were performed with a modified Ussing chamber system. Isolated tissue specimens were incubated in Ringer's solution, in amiloride and/or bumetanide, or in dimethyl sulfoxide (DMSO).
Under control conditions, i.e. when all the experimental fluids were Ringer's solution, the PD and R values of the rabbit cecum and colon were similar, while during mechanical stimulation, dPD of the colon was twice as high as that of the cecum. Addition of amiloride and/or bumetanide to all experimental fluids diminished the electrophysiological parameters of both tissues. DMSO added to all experimental fluids significantly diminished the values of the electrophysiological parameters of the cecum. Addition of amiloride to the stimulation fluid only diminished the PD and dPD values in the colon, whereas addition of bumetanide to the stimulation fluid only diminished the PD and dPD values in the cecum. It was found that the PD and dPD values of the rabbit cecum depend primarily on chloride ion transport, while those of the colon depend on sodium ion transport.
本研究的目的是比较阿米洛利和布美他尼对兔离体盲肠和结肠壁的基线跨上皮电位差（PD）以及机械刺激期间PD变化（dPD）的影响。
材料/方法：实验采用改良的尤斯灌流室系统进行。将离体组织标本置于林格氏液、阿米洛利和/或布美他尼中，或二甲基亚砜（DMSO）中孵育。
在对照条件下，即所有实验液均为林格氏液时，兔盲肠和结肠的PD和R值相似，而在机械刺激期间，结肠的dPD是盲肠的两倍。向所有实验液中添加阿米洛利和/或布美他尼会降低两种组织的电生理参数。向所有实验液中添加DMSO会显著降低盲肠的电生理参数值。仅向刺激液中添加阿米洛利会降低结肠中的PD和dPD值，而仅向刺激液中添加布美他尼会降低盲肠中的PD和dPD值。研究发现，兔盲肠的PD和dPD值主要取决于氯离子转运，而结肠的则取决于钠离子转运。
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