Careri Maria, Corradini Claudio, Elviri Lisa, Nicoletti Isabella, Zagnoni Ingrid
Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Parco Area delle Scienze, 17/A, 43100 Parma, Italy
J Agric Food Chem. 2003 Aug 27;51(18):5226-31. doi: 10.1021/jf034149g.
A simple and fast reversed-phase HPLC method using diode array detection was developed and validated for the simultaneous determination of trans-resveratrol and quercetin in Sicilian red wine from the Nero d'Avola red grape variety. Investigation was also extended to the quantitative determination of resveratrol and quercetin in grape skins and winemaking byproducts obtained from the same cultivar. Samples were eluted using a C18 narrow-bore column under isocratic conditions in less than 20 min. Quantification of trans-resveratrol and quercetin in red wine was performed without any sample pretreatment, whereas the determination of these phenolic compounds in grape skins and wine pomage required a solvent extraction procedure. Linearity was demonstrated over the 0.39-12.5 and 0.45-57.6 microg/mL range for trans-resveratrol and quercetin, respectively. Detection limits in real samples were in the low ppm level (0.07 mg/L for trans-resveratrol and 0.12 mg/L for quercetin). The HPLC-UV/DAD method was applied for the routine analyses of red wine and grape skin and winemaking byproduct extracts to evaluate their trans-resveratrol and quercetin content. In particular, a very high content of quercetin was found in wine pomace, suggesting the use of this wine byproduct as a potential source of this health-promoting phenolic compound.
建立了一种简单快速的反相高效液相色谱法，采用二极管阵列检测，用于同时测定来自黑达沃拉红葡萄品种的西西里红葡萄酒中的反式白藜芦醇和槲皮素。研究还扩展到对同一品种葡萄皮和酿酒副产物中白藜芦醇和槲皮素的定量测定。样品在等度条件下使用C18窄孔柱在不到20分钟内洗脱。红葡萄酒中反式白藜芦醇和槲皮素的定量无需任何样品预处理，而葡萄皮和酒渣中这些酚类化合物的测定需要溶剂萃取程序。反式白藜芦醇和槲皮素的线性范围分别为0.39 - 12.5和0.45 - 57.6μg/mL。实际样品中的检测限处于低ppm水平（反式白藜芦醇为0.07mg/L，槲皮素为0.12mg/L）。高效液相色谱 - 紫外/二极管阵列检测法用于红葡萄酒、葡萄皮和酿酒副产物提取物的常规分析，以评估它们的反式白藜芦醇和槲皮素含量。特别是，在酒渣中发现了非常高含量的槲皮素，表明这种葡萄酒副产物可作为这种促进健康的酚类化合物的潜在来源。
