Careri Maria, Corradini Claudio, Elviri Lisa, Nicoletti Isabella, Zagnoni Ingrid
Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Parco Area delle Scienze 17/A, I-43100 Parma, Italy.
J Agric Food Chem. 2004 Nov 17;52(23):6868-74. doi: 10.1021/jf049219d.
The application of liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) was investigated for the analysis of trans-resveratrol in red wine, grape skin, grape pomace, and winemaking byproducts. Chromatographic elution performed under isocratic reversed-phase conditions using a C18 narrow-bore LC column allowed retention times lower than 12 min to be obtained. Qualitative analysis was performed in negative-ion (NI) full-scan and product-ion scan acquisition modes, whereas method validation in terms of linearity, detection limits, accuracy, and robustness was carried out under NI selected reaction monitoring conditions. The matrix-matched detection limit was calculated in the low parts per billion range (10 microg/L). Results of the application of the method to red wine, grape, and winemaking byproduct samples were compared with those obtained using an LC-UV/DAD technique. Determination of trans-resveratrol in the samples investigated showed that the highest concentration was found in red wine, whereas wine made from grape pomace contained the lowest content.
研究了液相色谱 - 电喷雾串联质谱法（LC - ESI - MS/MS）在分析红酒、葡萄皮、葡萄渣及葡萄酒酿造副产物中反式白藜芦醇的应用。使用C18窄径液相色谱柱在等度反相条件下进行色谱洗脱，可获得低于12分钟的保留时间。定性分析在负离子（NI）全扫描和产物离子扫描采集模式下进行，而方法的线性、检测限、准确度和稳健性等验证则在NI选择反应监测条件下开展。基质匹配检测限在十亿分之几的低浓度范围（10微克/升）内计算得出。将该方法应用于红酒、葡萄及葡萄酒酿造副产物样品的结果与使用LC - UV/DAD技术获得的结果进行了比较。在所研究的样品中反式白藜芦醇的测定结果表明，红酒中的浓度最高，而由葡萄渣酿造的葡萄酒中含量最低。
