Elezi Ornel, Kourkoutas Yiannis, Koutinas Athanasios A, Kanellaki Maria, Bezirtzoglou Eugenia, Barnett Y A, Nigam Poonam
Food Biotechnology Group, Section of Analytical Environmental and Applied Chemistry, Department of Chemistry, University of Patras, GR-26500 Patras, Greece.
J Agric Food Chem. 2003 Aug 27;51(18):5285-9. doi: 10.1021/jf0262211.
Improvements in yield and productivity in lactic acid fermentation by Lactobaccilus brevis cells immobilized on delignified cellulosic (DC) material are reported. The system proved to be more efficient in comparison with the work reported by other workers. Yields of 80 and 100% conversion using glucose were obtained at 30 degrees C in 1 day of fermentation time. Lactic acid fermentation using whey as substrate was obtained at 30 degrees C in 1-1.5 days, resulting in 70% yield, whereas the remaining lactose in whey was converted to alcohol byproduct, leading to a 90% lactose exploitation and 100% conversion. Cell immobilization of L. brevis on DC material was proved by its reuses in repeated batch fermentations and through electron microscopy. A series of 10 repeated batch fermentations without any loss in cell activity showed a tendency for high operational stability. The presence of DC material resulted in a drastic drop of the fermentation time from 48 to 13 h.
据报道，固定在脱木质纤维素（DC）材料上的短乳杆菌细胞在乳酸发酵中的产量和生产率有所提高。与其他研究人员报道的工作相比，该系统被证明更有效。在30℃下发酵1天，使用葡萄糖的转化率分别达到80%和100%。以乳清为底物的乳酸发酵在30℃下进行1 - 1.5天，产率为70%，而乳清中剩余的乳糖转化为酒精副产物，乳糖利用率达到90%，转化率为100%。通过在重复分批发酵中的再利用以及电子显微镜观察，证明了短乳杆菌在DC材料上的细胞固定化。一系列10次重复分批发酵中细胞活性没有任何损失，显示出较高的操作稳定性。DC材料的存在使发酵时间从48小时急剧缩短至13小时。
