Zhu Lei, Lukeman Philip S, Canary James W, Seeman Nadrian C
Department of Chemistry, New York University, New York, New York 10003, USA.
J Am Chem Soc. 2003 Aug 27;125(34):10178-9. doi: 10.1021/ja035186r.
The synthesis of DNA/nylon ladder oligomers is described. Three stages of the development are addressed: the synthesis of 2'-beta-substituted phosphoramidites, the deprotection/purification protocols of ODNs modified with both amino and carboxyl groups, and amide bond-forming reactions on the ODNs. The established technology and the novel DNA-based ladder oligomer structure opens a pathway to the synthesis of topological molecular objects and networks templated by DNA through versatile DNA nanotechnology. The DNA-based ladder oligomers may find application in the antisense area.
描述了DNA/尼龙梯形寡聚物的合成。阐述了三个发展阶段：2'-β-取代亚磷酰胺的合成、同时带有氨基和羧基修饰的寡核苷酸（ODN）的脱保护/纯化方案，以及ODN上的酰胺键形成反应。已建立的技术和新型基于DNA的梯形寡聚物结构为通过通用的DNA纳米技术合成以DNA为模板的拓扑分子对象和网络开辟了一条途径。基于DNA的梯形寡聚物可能在反义领域找到应用。
