DNA及附加负载在阵列碳纳米管上的位点特异性组装。

Site-specific assembly of DNA and appended cargo on arrayed carbon nanotubes.

作者信息

Taft Bradford J, Lazareck Adam D, Withey Gary D, Yin Aijun, Xu J M, Kelley Shana O

机构信息

Department of Chemistry, Boston College, Chestnut Hill, MA 02467, USA.

出版信息

J Am Chem Soc. 2004 Oct 13;126(40):12750-1. doi: 10.1021/ja045543d.

DOI:10.1021/ja045543d

PMID:15469250

Abstract

We describe a strategy that permits discrete regions of arrayed carbon nanotubes (CNTs) to be functionalized simultaneously and specifically with DNA oligonucleotides. The different chemical properties of two regions on single CNTs and orthogonal chemical coupling strategies have been exploited to derivatize CNTs within highly ordered arrays with multiple DNA sequences. Through duplex hybridization, we then targeted different DNA sequences with appended metal nanoparticles to distinct sites on the CNT architecture with precise spatial control. The materials generated from these studies represent the first CNTs with bipartite functionalization. The approach described provides a high level of precision in parallel and directed assembly of DNA sequences and appended cargo and is useful for the preparation of novel hybrid bionanomaterials.

摘要

我们描述了一种策略，该策略允许排列的碳纳米管（CNT）的离散区域同时且特异性地用DNA寡核苷酸进行功能化。利用单个碳纳米管上两个区域的不同化学性质以及正交化学偶联策略，用多个DNA序列对高度有序阵列中的碳纳米管进行衍生化。然后，通过双链杂交，我们将带有附加金属纳米颗粒的不同DNA序列以精确的空间控制靶向到碳纳米管结构上的不同位点。这些研究产生的材料代表了首批具有双功能化的碳纳米管。所描述的方法在DNA序列和附加货物的平行和定向组装方面提供了高度的精确性，并且可用于制备新型混合生物纳米材料。