快速生长分枝杆菌中β-内酰胺酶的等电聚焦模式。

Isoelectric focusing patterns of beta-lactamases in the rapidly growing mycobacteria.

作者信息

Zhang Y, Wallace R J, Steingrube V A, Brown B A, Nash R, Silcox A, Tsukamura M

机构信息

Department of Microbiology, University of Texas Health Center, Tyler 75710.

出版信息

Tuber Lung Dis. 1992 Dec;73(6):337-44. doi: 10.1016/0962-8479(92)90037-k.

DOI:10.1016/0962-8479(92)90037-k

PMID:1292713

Abstract

beta-lactamases from 259 strains of rapidly growing mycobacteria that included the third biovariant complex of Mycobacterium fortuitum, M. peregrinum, M. abscessus, M. chelonae, the M. chelonae-like organisms (MCLO), and M. smegmatis were analyzed by isoelectric focusing (IEF). All isolates produced acidic beta-lactamases with major band isoelectric points (pIs) between 4.4 and 6.0. Each of the 6 taxonomic groups exhibited 1 or 2 characteristic beta-lactamase IEF patterns. Heterogeneity among IEF patterns was evident in 5 of the 6 groups, however, and was greatest among the third biovariant complex of M. fortuitum. beta-lactamase patterns correlated with previously identified taxonomic subgroups of M. smegmatis and the third biovariant complex of M. fortuitum. beta-lactamase IEF analysis of MCLO strains isolated from two outbreaks demonstrated its possible usefulness for epidemiologic evaluation.

摘要

通过等电聚焦（IEF）分析了来自259株快速生长分枝杆菌的β-内酰胺酶，这些分枝杆菌包括偶然分枝杆菌的第三生物变种复合体、龟分枝杆菌、脓肿分枝杆菌、耻垢分枝杆菌、类龟分枝杆菌（MCLO）以及龟分枝杆菌样菌。所有分离株均产生酸性β-内酰胺酶，主要条带的等电点（pI）在4.4至6.0之间。6个分类学组中的每一组都呈现出1种或2种特征性的β-内酰胺酶IEF图谱。然而，6个组中有5个组的IEF图谱存在明显的异质性，其中偶然分枝杆菌的第三生物变种复合体中的异质性最大。β-内酰胺酶图谱与耻垢分枝杆菌和偶然分枝杆菌的第三生物变种复合体先前确定的分类学子组相关。对从两次暴发中分离出的MCLO菌株进行的β-内酰胺酶IEF分析表明，其在流行病学评估中可能有用。