Wallace Richard J, Brown-Elliott Barbara A, Brown June, Steigerwalt Arnold G, Hall Leslie, Woods Gail, Cloud Joann, Mann Linda, Wilson Rebecca, Crist Christopher, Jost Kenneth C, Byrer Dorothy E, Tang Jane, Cooper Jason, Stamenova Elena, Campbell Brian, Wolfe Joyce, Turenne Christine
The University of Texas Health Center, Department of Microbiology, 11937 US Highway 271, Tyler, TX 75708, USA.
J Clin Microbiol. 2005 Dec;43(12):5925-35. doi: 10.1128/JCM.43.12.5925-5935.2005.
Mycobacterium peregrinum consists of two taxa: types I and II. We evaluated 43 clinical type II strains from throughout the United States. They were responsible for soft-tissue and bone infections, catheter-related infections, and possible pneumonitis. By carbohydrate utilization, they were indistinguishable from type I strains, being D-mannitol and trehalose positive. However, they had a distinct susceptibility pattern that included intermediate ciprofloxacin MICs but low clarithromycin and doxycycline MICs of < or =1 microg/ml. These features were also shared by reference isolates of Mycobacterium senegalense from African bovine cases of "farcy." By 16S rRNA gene sequencing, the type II isolates shared 100% sequence identity with M. senegalense. Partial sequencing of the type II hsp65 gene (441 bp) revealed four sequevars showing > or =98.4% identity with each other and > or =98.6% identity with the sequence of five bovine strains of M. senegalense. There was < or =97.1% identity with M. peregrinum type I isolates and other Mycobacterium fortuitum group species. Sequencing of additional gene targets including the 16S-23S rDNA internal transcribed spacer region and the rpoB gene (partial sequence) revealed a similar phylogenetic grouping. DNA-DNA hybridization showed 76 to 99% relatedness between the bovine and human strains. These studies demonstrate that type II isolates are not isolates of M. peregrinum but represent human strains of M. senegalense. This study is the first to demonstrate this species as a human pathogen. Representative human M. senegalense strains include ATCC 35755 and newly submitted strains ATCC BAA-849, ATCC BAA-850, and ATCC BAA-851.
I型和II型。我们评估了来自美国各地的43株临床II型菌株。它们可引起软组织和骨感染、导管相关感染以及可能的肺炎。通过碳水化合物利用情况，它们与I型菌株无法区分，D - 甘露醇和海藻糖呈阳性。然而，它们具有独特的药敏模式，包括环丙沙星MIC值为中等，但克拉霉素和强力霉素的MIC值较低，≤1微克/毫升。来自非洲牛“马皮疽”病例的塞内加尔分枝杆菌参考菌株也具有这些特征。通过16S rRNA基因测序，II型分离株与塞内加尔分枝杆菌的序列同一性为100%。II型hsp65基因（441 bp）的部分测序显示有四个序列变种，它们彼此之间的同一性≥98.4%，与五株塞内加尔分枝杆菌牛菌株的序列同一性≥98.6%。与海分枝杆菌I型分离株和其他偶然分枝杆菌属物种的同一性≤97.1%。对包括16S - 23S rDNA内转录间隔区和rpoB基因（部分序列）在内的其他基因靶点进行测序，显示出相似的系统发育分组。DNA - DNA杂交显示牛菌株和人菌株之间的相关性为76%至99%。这些研究表明，II型分离株不是海分枝杆菌的分离株，而是代表塞内加尔分枝杆菌的人菌株。本研究首次证明该物种是一种人类病原体。代表性的人源塞内加尔分枝杆菌菌株包括ATCC 35755以及新提交的菌株ATCC BAA - 849、ATCC BAA - 850和ATCC BAA - 851。
