Sparks J, Ross G W
Tubercle. 1981 Dec;62(4):289-93. doi: 10.1016/s0041-3879(81)80011-6.
Analytical IEF has been used to give a direct visual comparison of beta-lactamases, down to extremely low levels, from very crude intracellular preparations. Identity and non-identity of strains could be proved by the pattern of bands produced by their beta-lactamases. Mycobacterial species gave a variety of beta-lactamase patterns. Identity was established between some strains of M. chelonei. IEF distinguished between enzymes within both the chelonei and abscessus sub-species that could not be differentiated by other methods. This technique could provide a means of identifying the source of a M. chelonei infection.
分析性等电聚焦已被用于对来自非常粗的细胞内制剂中的β-内酰胺酶进行直接视觉比较，直至极低水平。菌株的同一性和非同一性可以通过其β-内酰胺酶产生的条带模式来证明。分枝杆菌属物种呈现出多种β-内酰胺酶模式。在某些龟分枝杆菌菌株之间确定了同一性。等电聚焦区分了龟分枝杆菌亚种和脓肿分枝杆菌亚种内其他方法无法区分的酶。该技术可以提供一种鉴定龟分枝杆菌感染来源的方法。
