Deshpande R G, Khan M B, Navalkar G
Department of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, Georgia 30310.
Tuber Lung Dis. 1992 Dec;73(6):356-61. doi: 10.1016/0962-8479(92)90040-q.
Sonicates of several Mycobacterium avium complex (MAC) strains isolated from acquired immunodeficiency syndrome (AIDS) patients were characterized in order to study the prominent antigens of these strains. Sonicates of 6-week-old cultures were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. A major 12 kDa glycoprotein antigen was observed in all the sonicates along with other proteins ranging up to 100 kDa. Western blotting, using the 12 kDa M. leprae 'specific' murine monoclonal antibody (MAb) MLO6, indicated the presence of a determinant in the 12 kDa antigen (in all the MAC isolates studied) which was immunologically cross-reactive with the 12 kDa antigen of M. leprae. The transparent variant of MAC 101 also demonstrated MLO6 reactivity while the opaque variant did not. Polyclonal antiserum raised against MAC 101 sonicate reacted with all the MAC isolates in immunodiffusion. These observations point to the cross-reactivity between these strains and suggest that they possess a M. leprae 'specific' determinant on a cross-reacting component which could be involved in virulence.
为了研究从获得性免疫缺陷综合征（AIDS）患者中分离出的几种鸟分枝杆菌复合群（MAC）菌株的主要抗原，对这些菌株的超声裂解物进行了表征。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹法分析了6周龄培养物的超声裂解物。在所有超声裂解物中均观察到一种主要的12 kDa糖蛋白抗原以及其他分子量高达100 kDa的蛋白质。使用12 kDa麻风分枝杆菌“特异性”鼠单克隆抗体（MAb）MLO6进行蛋白质印迹分析表明，在12 kDa抗原中存在一个决定簇（在所研究的所有MAC分离株中），该决定簇与麻风分枝杆菌的12 kDa抗原具有免疫交叉反应性。MAC 101的透明变体也表现出MLO6反应性，而不透明变体则没有。针对MAC 101超声裂解物产生的多克隆抗血清在免疫扩散中与所有MAC分离株发生反应。这些观察结果表明这些菌株之间存在交叉反应性，并表明它们在一个可能与毒力有关的交叉反应成分上具有麻风分枝杆菌“特异性”决定簇。
