[KB细胞中期染色体的分离与纯化技术]
[Technique for isolation and purification of metaphase chromosomes of KB cells].
作者信息
Deminatti M, Laï J L, Desbiens X, Jacqueloot N
出版信息
C R Seances Soc Biol Fil. 1975;169(4):981-6.
PMID:129274
Abstract
Metaphase chromosomes were isolated from KB cells according to Salzman and Mendelsohn's method. Whole-mount electronic microscopic preparations of chromosomes in situ, after isolation and after purification showed that the morphological patterns of this material are preserved in spite of physical and chemical treatments (pH 3).
摘要
根据萨尔兹曼和门德尔松的方法，从KB细胞中分离出中期染色体。对分离后和纯化后的染色体进行整装原位电子显微镜观察，结果显示，尽管经过了物理和化学处理（pH值为3），该材料的形态模式仍得以保留。
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