Liao Xiang, Ying Tianyi, Wang Hengliang, Wang Jie, Shi Zhaoxing, Feng Erling, Wei Kaihua, Wang Yuelan, Zhang Xuemin, Huang Liuyu, Su Guofu, Huang Peitang
Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing 100071, PR China.
Electrophoresis. 2003 Aug;24(16):2864-82. doi: 10.1002/elps.200305519.
Shigella flexneri is a Gram-negative facultatively intracellular pathogen responsible for bacillary dysentery in humans. In this study, extracellular proteins from the culture medium and whole cell proteins in cellular extracts of S. flexneri 2a strain 2457T were examined by two-dimensional (2-D) gel electrophoresis using immobilized pH gradient (IPG) technology. Proteins were identified by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) in combination with Mascot search program. In total, among the 488 proteins spots processed, 388 proteins were identified. The identified proteins represented 169 genes. By comparing results of Mascot search against databases of Escherichia coli and genomes of S. flexneri 2a, one S. flexneri-specific protein was identified and one possible gap was found in 2457T genome sequences. Although this proteome map is still incomplete, it is already a useful reference for future studies involving pathogenicity, vaccine development, design of novel antibacterial drugs, etc. Proteome maps and a table of all identified proteins are available on the internet at www.proteomics.com.cn.
福氏志贺菌是一种革兰氏阴性兼性细胞内病原体，可导致人类患细菌性痢疾。在本研究中，使用固定化pH梯度（IPG）技术通过二维（2-D）凝胶电泳对福氏志贺菌2a菌株2457T培养基中的细胞外蛋白质和细胞提取物中的全细胞蛋白质进行了检测。通过基质辅助激光解吸/电离质谱（MALDI-MS）结合Mascot搜索程序对蛋白质进行鉴定。总共，在处理的488个蛋白质斑点中，鉴定出388种蛋白质。这些鉴定出的蛋白质代表了169个基因。通过将Mascot搜索结果与大肠杆菌数据库和福氏志贺菌2a基因组进行比较，鉴定出一种福氏志贺菌特异性蛋白质，并在2457T基因组序列中发现了一个可能的缺口。尽管该蛋白质组图谱仍不完整，但它已经是未来涉及致病性、疫苗开发、新型抗菌药物设计等研究的有用参考。蛋白质组图谱和所有鉴定出的蛋白质表格可在互联网上的www.proteomics.com.cn获取。
