Ying Tianyi, Wang Hengliang, Li Mingzhu, Wang Junjun, Wang Jie, Shi Zhaoxing, Feng Erling, Liu Xiankai, Su Guofu, Wei Kaihua, Zhang Xuemin, Huang Peitang, Huang Liuyu
Beijing Institute of Biotechnology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
Proteomics. 2005 Dec;5(18):4777-93. doi: 10.1002/pmic.200401326.
Shigella flexneri 2a is an important pathogen causing bacillary dysentery in humans. In order to investigate any potential vaccine candidate proteins present in outer membrane proteins (OMPs) and extracellular proteins of S. flexneri 2a 2457T, we use the proteome mapping and database analyzing techniques. A subproteome map and database of OMPs were established first. One hundred and nine of the total 126 marked spots were cut out and processed to MALDI-TOF-MS and PMF. Eighty-seven spots were identified and they represented 55 OMP entries. Furthermore, immunoproteomics analysis of OMPs and extracellular proteins were performed. Total of 34 immunoreactive spots were identified, in which 22 and 12 were from OMPs and extracellular proteins, respectively. Eight novel antigens were found and some of these antigens may be potential vaccine candidate proteins. These results are useful for future studying of pathogenicity, vaccine, and novel antibacterial drugs. Maps and tables of all identified proteins are available on the Internet at www.proteomics.com.cn.
福氏志贺菌2a是引起人类细菌性痢疾的重要病原菌。为了研究福氏志贺菌2a 2457T外膜蛋白（OMPs）和细胞外蛋白中存在的任何潜在疫苗候选蛋白，我们采用了蛋白质组图谱和数据库分析技术。首先建立了OMPs的亚蛋白质组图谱和数据库。从总共126个标记斑点中切出109个，进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和肽质量指纹图谱（PMF）分析。鉴定出87个斑点，它们代表55个OMP条目。此外，还对OMPs和细胞外蛋白进行了免疫蛋白质组学分析。共鉴定出34个免疫反应性斑点，其中分别有22个和12个来自OMPs和细胞外蛋白。发现了8种新抗原，其中一些抗原可能是潜在的疫苗候选蛋白。这些结果对未来致病性、疫苗和新型抗菌药物的研究具有重要意义。所有鉴定出的蛋白质的图谱和表格可在互联网上www.proteomics.com.cn获取。
