Teanpaisan R, Douglas C W, Eley A R, Walsh T F
Department of Oral Pathology, University of Sheffield, UK.
J Periodontal Res. 1996 Aug;31(6):423-32. doi: 10.1111/j.1600-0765.1996.tb00511.x.
Black-pigmented anaerobes have been implicated as major pathogens in the aetiology of adult periodontitis but these organisms are also found in healthy sites. This study aimed to examine the relationship between genotypes of black-pigmented anaerobes and disease status of periodontal sites using restriction endonuclease analysis (REA) and ribotyping. The main black-pigmented species recovered from sites were Porphyromonas gingivalis, Prevotella intermedia and Prevotella nigrescens. Each of the 58 subjects investigated harboured distinct genotypes of these three species. Most subjects appeared to be colonized by a single genotype of P. gingivalis and Pr. intermedia, whereas multiple types of Pr. nigrescens colonized many individuals. Plasmids were only found in a few Pr. nigrescens strains. No association was found between the disease status of sites and any specific or group of genotypes of either species or presence of a plasmid. Since the same genotypes of P. gingivalis, Pr. intermedia and Pr. nigrescens were found at both diseased and non-diseased sites in a subject, adult periodontitis is not explained by the presence of specially virulent clones of these organisms. Their role in periodontitis, therefore, is likely to be opportunistic.
黑色色素厌氧菌被认为是成人牙周炎病因中的主要病原体，但这些微生物也存在于健康部位。本研究旨在利用限制性内切酶分析（REA）和核糖分型技术，研究黑色色素厌氧菌的基因型与牙周部位疾病状态之间的关系。从这些部位分离出的主要黑色色素菌种为牙龈卟啉单胞菌、中间普氏菌和变黑普氏菌。在接受调查的58名受试者中，每个人体内这三种菌种都具有不同的基因型。大多数受试者似乎仅被单一基因型的牙龈卟啉单胞菌和中间普氏菌定植，而多种类型的变黑普氏菌定植于许多个体。仅在少数变黑普氏菌菌株中发现了质粒。未发现部位的疾病状态与任何一种菌种的特定基因型或基因型组合，或质粒的存在之间存在关联。由于在同一受试者的患病部位和非患病部位都发现了相同基因型的牙龈卟啉单胞菌、中间普氏菌和变黑普氏菌，因此成人牙周炎不能用这些微生物中特别具有毒力的克隆的存在来解释。因此，它们在牙周炎中的作用可能是机会性的。
