Sheth N A, Teni T R, Mundle S D
Endocrinology Unit, Cancer Research Institute, Bombay, India.
Indian J Exp Biol. 1992 Nov;30(11):1024-9.
Present studies deal with the role of inhibin in proliferation and growth. The effect of inhibin on incorporation of 3H-thymidine in prostatic DNA in vivo as well as by NRK-49F and Balb/c3T3 cell lines in vitro, was investigated. Also studied the immunocytochemical localization of inhibin in normally proliferating and differentiated tissues of human prostate and endometrium. The in vivo studies revealed a suppression of 3H-thymidine uptake both in ventral (33%) and dorsolateral (26%) lobes of rat prostate. Interestingly, the histology of inhibin treated rat prostate manifested amidst the epithelial lining, an appearance of apoptotic bodies which are considered to be indicative of cell death. Further, the immunocytochemical studies for localization of inhibin showed intense staining in the differentiated human prostate and endometrium as compared to the respective proliferative tissues. Is inhibin kept suppressed in these proliferating tissues, because it is antiproliferative? The present in vitro experiments demonstrated that, at low inhibin concentrations, the incorporation of 3H-thymidine is stimulated while at higher doses it is suppressed. Thus, it is clear that prostatic inhibin seems to have a concentration-dependent dual role in the regulation of DNA synthesis.
目前的研究涉及抑制素在增殖和生长中的作用。研究了抑制素对体内前列腺DNA中3H-胸腺嘧啶掺入的影响，以及对体外NRK-49F和Balb/c3T3细胞系的影响。还研究了抑制素在人前列腺和子宫内膜正常增殖和分化组织中的免疫细胞化学定位。体内研究显示，大鼠前列腺腹侧叶（33%）和背外侧叶（26%）的3H-胸腺嘧啶摄取均受到抑制。有趣的是，经抑制素处理的大鼠前列腺组织学显示，在上皮内衬中出现了凋亡小体，这被认为是细胞死亡的标志。此外，抑制素定位的免疫细胞化学研究表明，与各自的增殖组织相比，在分化的人前列腺和子宫内膜中染色强烈。抑制素在这些增殖组织中是否受到抑制，是因为它具有抗增殖作用？目前的体外实验表明，在低抑制素浓度下，3H-胸腺嘧啶的掺入受到刺激，而在高剂量下则受到抑制。因此，很明显，前列腺抑制素在DNA合成调节中似乎具有浓度依赖性的双重作用。
