Pistillo M P, Sguerso V, Ferrara G B
National Institute of Cancer Research, Genoa, Italy.
Hum Immunol. 1992 Dec;35(4):256-9. doi: 10.1016/0198-8859(92)90008-b.
In an attempt to develop a suitable model for increasing the yield of human anti-HLA mAbs, we have used mice with SCID for i.p. injection of two human-mouse heterohybridomas. HMP1 hybridoma secretes a DQB10201 allele-specific human mAb whereas HMP12 secretes a human mAb recognizing the DRB11101, 1102, 1103, and 1104 alleles. Both hybridomas could be grown in SCID mice as localized tumors with no apparent alteration in the morphology of the cells or in the immunoglobulin secretion. Ascitic fluid was produced that showed a 600- to 1000-fold increase in monoclonal antibody cytotoxic titer as compared with that obtained in tissue culture. HLA-DQB1* and DRB1* alleles recognized by ascites and supernatants from SCID-derived cultures were analyzed by microlymphocytotoxicity assay on a small panel of B-lymphoblastoid cell lines. The results show that HLA specificity was retained after in vivo passage.
为了开发一种合适的模型来提高人抗 HLA 单克隆抗体的产量，我们使用了 SCID 小鼠进行腹腔注射两种人-鼠异种杂交瘤。HMP1 杂交瘤分泌一种 DQB10201 等位基因特异性人单克隆抗体，而 HMP12 分泌一种识别 DRB11101、1102、1103 和 1104 等位基因的人单克隆抗体。两种杂交瘤都可以在 SCID 小鼠中作为局部肿瘤生长，细胞形态或免疫球蛋白分泌没有明显改变。产生的腹水显示单克隆抗体细胞毒性效价比在组织培养中获得的提高了 600 至 1000 倍。通过对一小部分 B 淋巴母细胞系进行微量淋巴细胞毒性试验，分析了 SCID 来源培养物的腹水和上清液所识别的 HLA-DQB1和 DRB1等位基因。结果表明，体内传代后 HLA 特异性得以保留。
