Kadoya K, Amano S, Inomata S, Tsunenaga M, Matsuzaki K, Oshima H, Tanabe M, Kumagai N, Nishiyama T
Shiseido Life Science Research Centre 2-12-1 Fukuura, Kanazawa-ku, Yokohama-shi, 236-8643, Japan.
Br J Dermatol. 2003 Aug;149(2):377-80. doi: 10.1046/j.1365-2133.2003.05382.x.
Cultured epidermal autographs (CEAs) are currently used as a coverage treatment for burn wounds, for disfiguring burn scars involving depigmentation and in restoring the elasticity of the skin. The advantage of CEAs is that epidermal sheets prepared from small skin pieces can be enlarged sufficiently to cover large burn areas.
We examined the correlation between recovery of skin texture, and elastic fibre formation and keratinocyte differentiation (assessed by immunohistochemistry) in CEAs used as replacement skin after tattoo excision in a Japanese patient.
The tattooed skin was excised down to the deep dermal layer and then CEA was transplanted onto the patient. The skin textures were evaluated by taking replicas of the skin surface, and histological changes of filaggrin, transglutaminase, involucrin, fibrillin and elastin in the autograft skin were examined by immunohistochemistry.
The skin texture improved with time after grafting the CEA, and appeared similar to that of normal skin at 39 months. Among keratinocyte differentiation markers, filaggrin recovered to a normal pattern at around 6 months, and transglutaminase did so at 39 months, whereas involucrin expression remained abnormal at 39 months. Fibrillin expression appeared similar to that of normal skin by 39 months, except for sparse candelabra-like structures of short fibres. Elastin expression remained at a low level throughout.
Our results show that the recovery of skin texture after application of CEAs following tattoo excision is associated with the normalization of epidermal differentiation markers, except involucrin, and with the regeneration of elastic fibres in the dermis.
培养的表皮自体移植片（CEA）目前被用作烧伤创面的覆盖治疗、用于涉及色素脱失的毁容性烧伤瘢痕以及恢复皮肤弹性。CEA的优点是由小块皮肤制备的表皮片可充分扩大以覆盖大面积烧伤区域。
我们在一名日本患者纹身切除后使用CEA作为替代皮肤的情况下，研究了皮肤质地恢复、弹性纤维形成与角质形成细胞分化（通过免疫组织化学评估）之间的相关性。
将纹身皮肤切除至真皮深层，然后将CEA移植到患者身上。通过取皮肤表面复制品评估皮肤质地，并通过免疫组织化学检查自体移植皮肤中丝聚合蛋白、转谷氨酰胺酶、内披蛋白、原纤蛋白和弹性蛋白的组织学变化。
移植CEA后，皮肤质地随时间改善，在39个月时与正常皮肤相似。在角质形成细胞分化标志物中，丝聚合蛋白在约6个月时恢复到正常模式，转谷氨酰胺酶在39个月时恢复正常，而内披蛋白表达在39个月时仍异常。到39个月时，原纤蛋白表达与正常皮肤相似，除了短纤维的稀疏烛台样结构。弹性蛋白表达始终处于低水平。
我们的结果表明，纹身切除后应用CEA后皮肤质地的恢复与除内披蛋白外的表皮分化标志物的正常化以及真皮中弹性纤维的再生有关。
