Otsuka M, Ajimi S, Kajiwara Y, Ogura S, Kakimoto K, Inai T, Tanaka H, Ohuchida A
Hita Research Laboratories, Chemicals Inspection and Testing Institute 3-822, Oita, Japan.
J Toxicol Sci. 1992 Nov;17 Suppl 3:269-81. doi: 10.2131/jts.17.supplementiii_269.
Prednisolone farnesylate (PNF) was tested for mutagenicity by Ames test using Salmonella typhimurium (TA100, TA1535, TA98, TA1537) and Escherichia coli (WP2 uvrA), for clastogenic activity in vitro by the chromosomal aberration test in a Chinese hamster fibroblast cell line (CHL), and for induction of micronuclei by the micronucleus test in male ICR mice. 1) In Ames test, PNF with and without metabolic activation showed no mutagenicity in any strains at any dose levels (312-5,000 micrograms/plate). 2) In the chromosomal aberration test, PNF with metabolic activation produced a slight increase in the incidence of structural chromosomal aberrations in CHL cells at 1,500 micrograms/ml. 3) In the micronucleus test, a single administration of PNF caused no significant increase of micronucleated polychromatic erythrocytes at any doses (250-2,000 mg/kg).
采用鼠伤寒沙门氏菌（TA100、TA1535、TA98、TA1537）和大肠杆菌（WP2 uvrA）的艾姆斯试验检测了法尼醇泼尼松龙（PNF）的致突变性，通过中国仓鼠成纤维细胞系（CHL）的染色体畸变试验检测了其体外致断裂活性，并通过雄性ICR小鼠的微核试验检测了其诱导微核的能力。1）在艾姆斯试验中，有或无代谢激活的PNF在任何剂量水平（312 - 5000微克/平板）下对任何菌株均无致突变性。2）在染色体畸变试验中，有代谢激活的PNF在1500微克/毫升时使CHL细胞中结构染色体畸变的发生率略有增加。3）在微核试验中，单次给予PNF在任何剂量（250 - 2000毫克/千克）下均未导致微核多染红细胞显著增加。
