Hirai O, Miyamae Y, Zaizen K, Miyamoto A, Takashima M, Hattori Y, Ohara K, Mine Y
Toxicology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.
J Toxicol Sci. 1994 May;19(2):89-96. doi: 10.2131/jts.19.2_89.
The genotoxicity of polyoxyethylene hydrogenated castor oil 60 (HCO-60) was examined in reverse mutation test in bacteria, chromosome aberration test in vitro and micronucleus test in mice. The in vitro tests were done with and without metabolic activation using rat liver microsomal fraction. HCO-60 did induce neither reverse mutation in Salmonella typhimurium TA100, TA98, TA1535, and TA1537 and in Escherichia coli WP2uvrA, nor chromosome aberrations in Chinese hamster V79 cells. In addition, no increase in micronucleated polychromatic erythrocytes was elicited in the bone marrow of BDF1 male and female mice. It is concluded that HCO-60 was not genotoxic in these in vitro and in vivo assays.
在细菌回复突变试验、体外染色体畸变试验及小鼠微核试验中检测了聚氧乙烯氢化蓖麻油60（HCO - 60）的遗传毒性。体外试验使用大鼠肝微粒体组分，分别在有和无代谢活化的条件下进行。HCO - 60在鼠伤寒沙门氏菌TA100、TA98、TA1535和TA1537以及大肠杆菌WP2uvrA中均未诱导回复突变，在中国仓鼠V79细胞中也未诱导染色体畸变。此外，BDF1雄性和雌性小鼠骨髓中多染红细胞的微核率也未增加。得出结论：在这些体外和体内试验中，HCO - 60无遗传毒性。
