多杀性巴氏杆菌的签名标签诱变鉴定出在小鼠和鸡中表现出不同毒力特征的突变体。
Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens.
作者信息
Harper Marina, Boyce John D, Wilkie Ian W, Adler Ben
机构信息
Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
出版信息
Infect Immun. 2003 Sep;71(9):5440-6. doi: 10.1128/IAI.71.9.5440-5446.2003.
Abstract
Pasteurella multocida is the causative agent of fowl cholera in birds. Signature-tagged mutagenesis (STM) was used to identify potential virulence factors in a mouse septicemia disease model and a chicken fowl cholera model. A library of P. multocida mutants was constructed with a modified Tn916 and screened for attenuation in both animal models. Mutants identified by the STM screening were confirmed as attenuated by competitive growth assays in both chickens and mice. Of the 15 mutants identified in the chicken model, only 5 were also attenuated in mice, showing for the first time the presence of host-specific virulence factors and indicating the importance of screening for attenuation in the natural host.
摘要
多杀性巴氏杆菌是禽类禽霍乱的病原体。利用签名标签诱变技术（STM）在小鼠败血症疾病模型和鸡禽霍乱模型中鉴定潜在的毒力因子。用改良的Tn916构建了一个多杀性巴氏杆菌突变体文库，并在两种动物模型中筛选减毒株。通过STM筛选鉴定出的突变体在鸡和小鼠中均通过竞争性生长试验确认为减毒株。在鸡模型中鉴定出的15个突变体中，只有5个在小鼠中也表现出减毒，首次证明了宿主特异性毒力因子的存在，并表明在天然宿主中筛选减毒株的重要性。
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