Avila R E, Samar M E, Ferraris R, Bonomi L
Facultad de Ciencias Médicas, U.N.C., IIa. Cátedra de Biología Celular, Histología y Embriología.
Rev Fac Cien Med Univ Nac Cordoba. 2001;58(1):49-55.
In a previous work we demonstrated the relationship between nerve fibers and nerve endings and interstitial cells (estrogen-producing cells) from the atrophic right ovary and the medulla in the left functioning ovary during embryogenesis in the chick, in ovo. Besides, the local production of neurotrophins by steroidogenic cells is probably involved in the control of ovarian innervation. The objective of the present study was to analyze ultrastructurally the innervation during the differentiation of chick ovary cultured with 17-beta-estradiol. Explants of right and left ovaries from seven to nineteen days in ovo development were cultured separately for 4 days in MEM (controls) or in the presence of 17-beta-estradiol (problems). In controls the electron microscopic examination of the innervation explants from chick embryo ovaries revealed that the interstitial cells are well innervated. Nerve fibres and nerve endings were observed in close contact with steroid-producing cells, a similar pattern of innervation that those of the fifteen days ovaries in ovo development. Problems cultured from seven days showed nerve fibres and nerve endings at difference to controls. These results in vitro suggest that innervation of the ovaries is controlled by indirect mechanism via the hypothalamic-pituitary system and local production factors. More experiments are necessary to confirm this results.
在之前的一项研究中，我们展示了鸡胚胎发育过程中，萎缩的右侧卵巢以及左侧功能正常卵巢髓质中的神经纤维、神经末梢与间质细胞（产生雌激素的细胞）之间的关系。此外，类固醇生成细胞局部产生神经营养因子可能参与了卵巢神经支配的调控。本研究的目的是对用17-β-雌二醇培养的鸡卵巢分化过程中的神经支配进行超微结构分析。将处于胚胎发育7至19天的左右卵巢外植体分别在MEM培养基（对照组）或17-β-雌二醇存在的条件下（实验组）培养4天。在对照组中，对鸡胚卵巢神经支配外植体的电子显微镜检查显示，间质细胞有丰富的神经支配。观察到神经纤维和神经末梢与类固醇生成细胞紧密接触，这种神经支配模式与胚胎发育15天的卵巢相似。7天开始培养的实验组与对照组相比，出现了神经纤维和神经末梢的差异。这些体外实验结果表明，卵巢的神经支配是通过下丘脑-垂体系统和局部产生的因子以间接机制进行调控的。需要更多实验来证实这一结果。
