Avila R E, Samar M E, de Fabro S P
Rev Fac Cien Med Univ Nac Cordoba. 1987;45(2):17-20.
Having studied the functioning left ovary and the atrophic right ovary of the chicken during its in ovo embryogeny, we decided to study its development in vitro. For that purpose the explants of left and right ovaries of 7 at 19 days of in ovo development were cultured in Eagle medium, supplemented by bovine fetal serum and lated processed for structural and ultrastructural studies. At every ages the left ovary showed a cortex and a medulla while the right ovary had no cortex. Epithelial cells were observed in the cortex, encircling ovocytes having typical Balbiani's bodies formed by mitochondria, Golgi aparatus and centriole. Some nuclei were in the meiotic prophase. The right ovary and the medulla of the left ovary failed to show the lacunar aspect characteristic in the ovo study. Conversely, cell cords were found, formed by epithelial cells and ovocytes which being very close to each other, gave the cord a compact aspect. Among the cords, groups of interstitial cells could be seen, which were more frequent in the cultures of older ova and in both ovaries. The left and the right ovaries, therefore, developed according to the genetic pattern. Notwithstanding, the left ovary medulla and the right ovary would be modulated by extragonadal factors which would be inactive when the gonads are not in their natural environment.
在研究了鸡胚在卵内胚胎发育过程中左卵巢的功能及萎缩的右卵巢后，我们决定研究其体外发育情况。为此，将19日龄鸡胚的左、右卵巢外植体在添加了牛胎血清的伊格尔培养基中培养，并进行后期的结构和超微结构研究。在各个年龄段，左卵巢均可见皮质和髓质，而右卵巢无皮质。在皮质中观察到上皮细胞，环绕着具有由线粒体、高尔基体和中心粒形成的典型巴尔比亚尼小体的卵母细胞。一些细胞核处于减数分裂前期。右卵巢和左卵巢的髓质在卵内研究中未表现出典型的腔隙特征。相反，发现了由上皮细胞和卵母细胞形成的细胞索，它们彼此非常靠近，使细胞索呈现紧密的外观。在细胞索之间，可以看到成群的间质细胞，在较老龄卵子的培养物以及两个卵巢中更为常见。因此，左、右卵巢均按照遗传模式发育。尽管如此，左卵巢髓质和右卵巢会受到性腺外因素的调节，当性腺不在其自然环境中时，这些因素将不发挥作用。
