Avila R E, Samar M E, de Fabro S P
II Cátedra de Histología, Embriología y Genética, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Rev Fac Cien Med Univ Nac Cordoba. 1991;49(2):7-12.
The purpose of the present work was to analyze the structural variations produced "in vitro" by hormones on the cell surfaces of the ovarian epithelium of the chick embryo, in relation to the growth of the left ovary and atrophy of the right one. Explants of both ovaries from embryos at 7 to 19 days of development were separately cultured for 4 days in MEM with 10% of fetal bovine serum (control), and plus 17-beta-estradiol, testosterone propionate, progesterone, FSH, LH or hCG added individually to cultures (experimental). The cultures were processed for their structural, ultrastructural and cytochemical study. In control cultures, membrane differentiations and mucosubstances were similar in both ovaries and at all ages to those in ovo. 17-beta-estradiol produced a greater development of microvilli, junctional complexes and mucin in the epithelial cells and interdigitations in the germ cells of the left ovary, while in the right gonad there was cell regression. Testosterone and progesterone evoked in the left gonad a response similar to that obtained with the estrogen, whereas in the right ovary no changes were observed with respect to controls. FSH led to cellular regression in both ovaries. Finally, with LH or hCG the changes produced in the left ovary were similar to those induced by the estrogen, and in the right one an increase of membrane differentiations and of mucosubstances related to them was found in comparison to controls.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是分析激素在体外对鸡胚卵巢上皮细胞表面产生的结构变化，及其与左卵巢生长和右卵巢萎缩的关系。将发育7至19天胚胎的双侧卵巢外植体分别在含有10%胎牛血清的MEM培养基中培养4天（对照组），并在培养物中分别添加17-β-雌二醇、丙酸睾酮、孕酮、促卵泡激素（FSH）、促黄体生成素（LH）或人绒毛膜促性腺激素（hCG）（实验组）。对培养物进行结构、超微结构和细胞化学研究。在对照培养物中，双侧卵巢在所有年龄段的膜分化和黏液物质与卵内情况相似。17-β-雌二醇使左卵巢上皮细胞中的微绒毛、连接复合体和黏蛋白有更大程度的发育，生殖细胞中有指状交叉，而右侧性腺出现细胞退化。睾酮和孕酮在左性腺引起的反应与雌激素相似，而右侧卵巢与对照组相比未观察到变化。FSH导致双侧卵巢细胞退化。最后，LH或hCG在左卵巢产生的变化与雌激素诱导的变化相似，而在右侧卵巢，与对照组相比，发现膜分化及其相关黏液物质增加。（摘要截断于250字）
