Takanami Yuichiro, Chida Masahiro, Hasebe Hayato, Sone Yukio, Suhara Shizuo
Tobacco Science Research Center, Japan Tobacco Inc, 6-2, Umegaoka, Aoba-ku, Yokohama, Kanagawa 227-8512, Japan.
J Chromatogr Sci. 2003 Jul;41(6):317-22. doi: 10.1093/chromsci/41.6.317.
Single puffs of cigarette smoke with a wide continuous range of volatility are directly analyzed using a new system. The system consists of a smoking machine, an online thermal desorption system (TDS), and a multidimensional gas chromatograph-mass spectrometer (MDGC-MS) system. The online TDS with the smoking machine collects the single-puff cigarette smoke with glass beads as the cryogenic adsorbent. The MDGC is composed of three capillary columns, Poraplot Q, and DB-WAX for separation and a deactivated capillary column for pressure balance, which enables simultaneous separation of the two different phases. The smoke desorbed from the TDS is divided into vapor and semivolatile phases and analyzed individually with each column by the MDGC. Thus, the system enables the overall analysis of the two phases simultaneously, including acetaldehyde and 1,4-benzenediol. This system also provides more appropriate analysis for compounds crossing the two phases such as toluene and pyridine. For the approach of introducing internal standards, a gas mixture of toluene-d(8) and o-xylene-d(10) is applied and the compounds are detected in the vapor and semivolatile phases, respectively.
使用一种新系统对具有广泛连续挥发性范围的单口香烟烟雾进行直接分析。该系统由一台吸烟机、一个在线热脱附系统（TDS）和一个多维气相色谱 - 质谱联用仪（MDGC - MS）系统组成。与吸烟机相连的在线TDS以玻璃珠作为低温吸附剂收集单口香烟烟雾。MDGC由三根毛细管柱、Poraplot Q和用于分离的DB - WAX以及一根用于压力平衡的去活毛细管柱组成，它能够同时分离两个不同的相。从TDS解吸出来的烟雾被分为气相和半挥发性相，并通过MDGC分别用每根柱子进行分析。因此，该系统能够同时对两个相进行全面分析，包括乙醛和1,4 - 苯二酚。该系统还能对跨越两相的化合物（如甲苯和吡啶）进行更合适的分析。对于引入内标的方法，使用甲苯 - d(8)和邻二甲苯 - d(10)的气体混合物，并分别在气相和半挥发性相中检测化合物。
