Kobayashi T, Yokota M, Mitani T, Akane A
Forensic Science Laboratory, Osaka Prefectural Police Headquarters, Osaka 541-0053, Japan.
Leg Med (Tokyo). 1999 Apr;1(2):68-75. doi: 10.1016/s1344-6223(99)80015-7.
Using commercially-available monoclonal antibodies (Bioclone, Neo Kokusai, Monoclonal Wako, Gamma Clone and Seraclone), ABO blood grouping of forensic specimens such as bloodstains, salivary stains, seminal stains, nails, hair and cerebral dura mater was performed with an absorption-elution test. Salivary stains, seminal stains and nails were not typed correctly using the antibodies other than Bioclone reagents, while precise grouping of bloodstains was performed using most antibodies. When hair and dura mater were tested, all of the antibodies induced weak or non-specific haemagglutination, hence correct grouping was not achieved. When the antibody solvents were displaced with 5-20% bovine serum albumin in saline, human serum of the group AB donor, or serum of chicken, sheep or bovine, titers of the reagents increased 2-8 times. Hair and dura mater were able to be typed using Bioclone reagents after solvent displacement with human AB or sheep serum, whereas displacement with the other solvents enhanced non-specific reactions.
使用市售单克隆抗体（Bioclone、Neo Kokusai、Monoclonal Wako、Gamma Clone和Seraclone），通过吸收-洗脱试验对血迹、唾液斑、精液斑、指甲、毛发和硬脑膜等法医标本进行ABO血型鉴定。除Bioclone试剂外，使用其他抗体对唾液斑、精液斑和指甲进行血型鉴定时结果不准确，而使用大多数抗体都能对血迹进行精确分型。对毛发和硬脑膜进行检测时，所有抗体均诱导出微弱或非特异性血凝反应，因此无法实现正确分型。当抗体溶剂被生理盐水、AB型供体人血清或鸡、羊或牛血清中的5-20%牛血清白蛋白替代时，试剂的效价提高了2-8倍。在用AB型人血清或羊血清替代溶剂后，使用Bioclone试剂能够对毛发和硬脑膜进行血型鉴定，而用其他溶剂替代则会增强非特异性反应。
