Ikematsu Kazuya, Tsuda Ryouichi, Kondo Toshikazu, Kondo Hisayoshi, Ozawa Kentaro, Ogawa Satoshi, Nakasono Ichiro
Division of Forensic Pathology and Science, Department of Translational Medical Sciences, Course of Medical and Dental Sciences, Graduate School of Biochemical Sciences, Nagasaki University, Nagasaki City, Nagasaki 852-8523, Japan.
Leg Med (Tokyo). 2003 Mar;5(1):15-9. doi: 10.1016/s1344-6223(02)00072-x.
The oxygen regulated protein 150-kDa (ORP-150) is only induced in hypoxic conditions. We performed an immunohistochemical and morphometrical study on the expression of ORP-150 in the brains of sudden infant death (SID) victims. The cerebral cortexes of 18 infants were used for this study. Each tissue section was incubated with anti-ORP-150 polyclonal antibodies and the number of ORP-150 positive cells was counted. In the cluster analysis, the 18 cases were classified into three groups (A-C groups). Group A was composed of six sudden infant death syndrome (SIDS) cases and its mean value of ORP-150 positive cells was 66.75+/-3.44, Group B (six severe respiratory infectious disease such as pneumonia and bronchitis including sepsis): 39.50+/-2.52 and Group C (five SIDS and one severe respiratory infectious disease): 16.00+/-2.92, respectively. These results might reflect chronic hypoxic condition before death, because ORP-150 is only induced when a hypoxic condition exist, but not acute hypoxia. And chronic hypoxic state is likely to be antecedent to SIDS. Therefore, immunohistochemical analysis of OPR-150 in the brain of SID cases may be very useful to differentiate between SIDS and acute asphyxia.
氧调节蛋白150千道尔顿（ORP - 150）仅在缺氧条件下被诱导产生。我们对婴儿猝死（SID）受害者大脑中ORP - 150的表达进行了免疫组织化学和形态计量学研究。本研究使用了18名婴儿的大脑皮质。每个组织切片与抗ORP - 150多克隆抗体孵育，并对ORP - 150阳性细胞数量进行计数。在聚类分析中，18例被分为三组（A - C组）。A组由6例婴儿猝死综合征（SIDS）病例组成，其ORP - 150阳性细胞的平均值为66.75±3.44；B组（6例患有严重呼吸道传染病如肺炎、支气管炎包括败血症）：39.50±2.52；C组（5例SIDS和1例严重呼吸道传染病）：分别为16.00±2.92。这些结果可能反映了死亡前的慢性缺氧状况，因为ORP - 150仅在存在缺氧条件时被诱导产生，而非急性缺氧。并且慢性缺氧状态很可能是SIDS的先兆。因此，对SID病例大脑中OPR - 150进行免疫组织化学分析可能对区分SIDS和急性窒息非常有用。
