Xue Yuying, Hieda Yoko, Kimura Kojiro, Nishiyama Takashi, Adachi Toshinori
Department of Legal Medicine, Shimane Medical University, 89-1 Enya, Izumo, Japan.
Leg Med (Tokyo). 2002 Dec;4(4):232-8. doi: 10.1016/s1344-6223(02)00036-6.
A sensitive and simple high-performance liquid chromatography assay for benzalkonium chloride (BZK) in biological samples was developed. The biological samples, spiked with domiphen used as an internal standard, were purified by solid-phase extraction. The major homologues of BZK in pharmaceutical products (C(12) and C(14)) were eluted at 24 and 36 min using a YMC-Pack CN column (4.6 x 250 mm, 5 microm) with a mobile phase, mixture of acetonitrile and sodium acetate buffer (48:52), and monitored at 254 nm. The most dominant component C(12) was selected as an indicator to quantify BZK, and adjustment was then done based on the proportion of C(12) in the BZK product. Good linearity was obtained in the range of 0.1-3 microg, and the limit of detection was 20 ng as a loaded amount on column. The recoveries of BZK in serum and tissues ranged from 54 to 90%. In a practical case, 0.16 microg/ml of BZK was quantified in serum collected several hours after accidental ingestion. The method is simple, sensitive and reliable for determining BZK levels in practical biological samples.
建立了一种灵敏且简便的生物样品中苯扎氯铵（BZK）的高效液相色谱检测方法。将添加了作为内标物的度米芬的生物样品通过固相萃取进行纯化。使用YMC-Pack CN柱（4.6×250 mm，5μm），以乙腈和醋酸钠缓冲液（48:52）的混合物作为流动相，在24和36分钟时洗脱药品中BZK的主要同系物（C(12)和C(14)），并在254 nm处进行监测。选择最主要的成分C(12)作为定量BZK的指标，然后根据其在BZK产品中的比例进行校正。在0.1 - 3μg范围内获得了良好的线性关系，柱上进样量的检测限为20 ng。BZK在血清和组织中的回收率为54%至90%。在一个实际案例中，意外摄入数小时后采集的血清中BZK的含量被定量为0.16μg/ml。该方法对于实际生物样品中BZK水平的测定简单、灵敏且可靠。
